PMID- 21847756
OWN - NLM
STAT- MEDLINE
DCOM- 20120131
LR  - 20211020
IS  - 1995-820X (Electronic)
IS  - 1674-0769 (Print)
IS  - 1995-820X (Linking)
VI  - 26
IP  - 4
DP  - 2011 Aug
TI  - Rapid and sensitive detection of PRRSV by a reverse transcription-loop-mediated 
      isothermal amplification assay.
PG  - 252-9
LID - 10.1007/s12250-011-3185-x [doi]
LID - 252
AB  - A real-time monitoring reverse transcription loop-mediated isothermal 
      amplification (RT-LAMP) assay was developed for the sensitive and specific 
      detection of prototypic, prevalent North American porcine reproductive and 
      respiratory syndrome virus (PRRSV) strains. As a higher sensitivity and 
      specificity method than reverse transcription polymerase chain reaction (RT-PCR), 
      the RT-LAMP method only used a turbidimeter, exhibited a detection limit 
      corresponding to a 10(-4) dilution of template RNA extracted from 250 muL of 10(5) 
      of the 50% tissue culture infective dose (TCID(50)) of PRRSV-containing cells, 
      and no cross-reactivity was observed with other related viruses including porcine 
      circovirus type 2, swine influenza virus, porcine rotavirus and classical swine 
      fever virus. From forty-two field samples, 33 samples in the RT-LAMP assay was 
      detected positive, whereas three of which were not detected by RT-PCR. 
      Furthermore, in 33 strains of PRRSV, an identical detection rate was observed 
      with the RT-LAMP assay to what were isolated using porcine alveolar macrophages. 
      These findings demonstrated that the RT-LAMP assay has potential clinical 
      applications for the detection of highly pathogenic PRRSV isolates, especially in 
      developing countries.
FAU - Zhang, Lei
AU  - Zhang L
AD  - China Institute of Veterinary Drug Control, Beijing 100081, China.
FAU - Liu, Ye-bing
AU  - Liu YB
FAU - Chen, Lei
AU  - Chen L
FAU - Wang, Jian-huan
AU  - Wang JH
FAU - Ning, Yi-bao
AU  - Ning YB
LA  - eng
PT  - Journal Article
DEP - 20110817
PL  - Netherlands
TA  - Virol Sin
JT  - Virologica Sinica
JID - 101514185
SB  - IM
MH  - Animals
MH  - *Nucleic Acid Amplification Techniques
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/*isolation & 
      purification
MH  - *Reverse Transcriptase Polymerase Chain Reaction
MH  - Swine
PMC - PMC7091248
EDAT- 2011/08/19 06:00
MHDA- 2012/02/01 06:00
PMCR- 2012/08/17
CRDT- 2011/08/18 06:00
PHST- 2011/01/24 00:00 [received]
PHST- 2011/05/11 00:00 [accepted]
PHST- 2011/08/18 06:00 [entrez]
PHST- 2011/08/19 06:00 [pubmed]
PHST- 2012/02/01 06:00 [medline]
PHST- 2012/08/17 00:00 [pmc-release]
AID - 3185 [pii]
AID - 10.1007/s12250-011-3185-x [doi]
PST - ppublish
SO  - Virol Sin. 2011 Aug;26(4):252-9. doi: 10.1007/s12250-011-3185-x. Epub 2011 Aug 
      17.