PMID- 21847775
OWN - NLM
STAT- MEDLINE
DCOM- 20120731
LR  - 20220408
IS  - 1573-2576 (Electronic)
IS  - 0360-3997 (Linking)
VI  - 35
IP  - 1
DP  - 2012 Feb
TI  - Proinflammatory CD14+CD16+ monocytes are associated with microinflammation in 
      patients with type 2 diabetes mellitus and diabetic nephropathy uremia.
PG  - 388-96
LID - 10.1007/s10753-011-9374-9 [doi]
AB  - Diabetic nephropathy (DN) is a major cause of type 2 diabetes mellitus (T2DM) 
      mortality. Innate immunity has been shown to be closely associated with the 
      occurrence and progression of T2DM-associated complications. In this study, we 
      investigated the expression of Toll-like receptor 4 (TLR4) and CD14(+)CD16(+) 
      monocytes in patients with T2DM and DN patients with uremia and TLR4 response to 
      lipopolysaccharide (LPS), and to further explore the potential effects of 
      inflammatory immune response in T2DM and DN uremia. Thirty DN patients with 
      uremia, 28 T2DM patients, and 20 healthy volunteers were enrolled for the 
      determination of CD14(+)CD16(+) fluorescence intensity and TLR4 expression on 
      monocytes by using peripheral blood flow cytometry. Serum C-reactive protein 
      (CRP) level was determined by using the immunoturbidimetry. Peripheral blood 
      mononuclear cells (PBMCs) were isolated and stimulated with LPS for 24 h. 
      monocytes were collected to detect NF-kappaB p65 and phosphorylated STAT5(p-STAT5) 
      expressions by using Western blotting. Supernatants were sampled for the 
      determination of interleukin-6 (IL-6) concentration by using ELISA. Compared to 
      normal control, T2DM patients and DN uremic patients had a significantly higher 
      CD14(+)CD16(+) fluorescence intensity, TLR4 expression, serum IL-6 and CRP level, 
      whilst these biomarkers were more upregulated in DN uremic patients than in T2DM 
      patients. Following the exposure to LPS, PBMCs showed a significant upregulation 
      in NF-kappaB-p65 and p-STAT5 expression and a remarked increase in Supernatants IL-6 
      level, in a positive correlation with disease severity. Our results suggest that 
      the disturbance in proinflammatory CD14(+)CD16(+) monocytes occurs in T2DM and DN 
      uremic patients. Such immunological dysfunction may be related to the activation 
      of TLR4/NF-kappaB and STAT5 signaling pathways underlying the immune abnormalities of 
      CD14(+)CD16(+) monocytes.
FAU - Yang, Mengxue
AU  - Yang M
AD  - Department of Nephrology, First Affiliated Hospital of ChongQing Medical 
      University, ChongQing, 400016, China. yangmengxue01@126.com
FAU - Gan, Hua
AU  - Gan H
FAU - Shen, Qing
AU  - Shen Q
FAU - Tang, Weixue
AU  - Tang W
FAU - Du, Xiaogang
AU  - Du X
FAU - Chen, Danyan
AU  - Chen D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Inflammation
JT  - Inflammation
JID - 7600105
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Receptors, IgG)
RN  - 0 (STAT5 Transcription Factor)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Transcription Factor RelA)
RN  - 9007-41-4 (C-Reactive Protein)
SB  - IM
MH  - C-Reactive Protein/analysis
MH  - Diabetes Mellitus, Type 2/*immunology/metabolism
MH  - Diabetic Neuropathies/*immunology/metabolism
MH  - Female
MH  - Humans
MH  - Inflammation/*immunology
MH  - Interleukin-6/biosynthesis
MH  - Lipopolysaccharide Receptors/*analysis
MH  - Lipopolysaccharides/immunology
MH  - Male
MH  - Monocytes/*immunology
MH  - NF-kappa B/metabolism
MH  - Receptors, IgG/*analysis
MH  - STAT5 Transcription Factor/metabolism
MH  - Signal Transduction
MH  - Toll-Like Receptor 4/metabolism
MH  - Transcription Factor RelA/biosynthesis
MH  - Uremia/immunology/metabolism
EDAT- 2011/08/19 06:00
MHDA- 2012/08/01 06:00
CRDT- 2011/08/18 06:00
PHST- 2011/08/18 06:00 [entrez]
PHST- 2011/08/19 06:00 [pubmed]
PHST- 2012/08/01 06:00 [medline]
AID - 10.1007/s10753-011-9374-9 [doi]
PST - ppublish
SO  - Inflammation. 2012 Feb;35(1):388-96. doi: 10.1007/s10753-011-9374-9.