PMID- 21848621
OWN - NLM
STAT- MEDLINE
DCOM- 20111215
LR  - 20110818
IS  - 1476-5829 (Electronic)
IS  - 1476-5810 (Linking)
VI  - 9
IP  - 3
DP  - 2011 Sep
TI  - Generation of recombinant antibody fragments that target canine dendritic cells 
      by phage display technology.
PG  - 183-95
LID - 10.1111/j.1476-5829.2010.00246.x [doi]
AB  - One of the main goals in cancer immunotherapy is the efficient activation of the 
      host immune system against tumour cells. Dendritic cells (DCs) can induce 
      specific anti-tumour immune responses in both experimental animal models and 
      humans. However, most preclinical studies using small animal models show only 
      limited correlation with studies carried out in clinical settings, whereas 
      laboratory dogs naturally develop tumours that are biologically and 
      histopathologically similar to their human counterparts. Here, we describe the 
      generation and characterization of recombinant antibodies against canine DCs, 
      isolated using the Tomlinson phage display system. We successfully isolated 
      highly specific single-chain variable fragment (scFv) antibodies in a sequential 
      three-step panning strategy involving depletion on canine peripheral blood 
      mononuclear cells followed by positive selection on native canine DCs. This 
      provides the basis for an antibody-based method for the immunological detection 
      and manipulation of DCs and for monitoring antigen-specific immune responses.
CI  - (c) 2010 Blackwell Publishing Ltd.
FAU - Fitting, J
AU  - Fitting J
AD  - Experimental Medicine and Immunotherapy, Department of Applied Medical 
      Engineering, Helmholtz Institute for Biomedical Engineering, University Hospital 
      RWTH Aachen, Aachen, Germany.
FAU - Killian, D
AU  - Killian D
FAU - Junghanss, C
AU  - Junghanss C
FAU - Willenbrock, S
AU  - Willenbrock S
FAU - Murua Escobar, H
AU  - Murua Escobar H
FAU - Lange, S
AU  - Lange S
FAU - Nolte, I
AU  - Nolte I
FAU - Barth, S
AU  - Barth S
FAU - Tur, M K
AU  - Tur MK
LA  - eng
SI  - GENBANK/HM560963
SI  - GENBANK/HM560964
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100930
PL  - England
TA  - Vet Comp Oncol
JT  - Veterinary and comparative oncology
JID - 101185242
RN  - 0 (Antigens, CD34)
RN  - 0 (Peptide Library)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Single-Chain Antibodies)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibody Affinity
MH  - Antigens, CD34/immunology
MH  - Dendritic Cells/*immunology
MH  - Dogs
MH  - Female
MH  - Leukocytes, Mononuclear/immunology
MH  - Male
MH  - Molecular Sequence Data
MH  - *Peptide Library
MH  - Recombinant Proteins/*immunology
MH  - Single-Chain Antibodies/immunology
EDAT- 2011/08/19 06:00
MHDA- 2011/12/16 06:00
CRDT- 2011/08/19 06:00
PHST- 2011/08/19 06:00 [entrez]
PHST- 2011/08/19 06:00 [pubmed]
PHST- 2011/12/16 06:00 [medline]
AID - 10.1111/j.1476-5829.2010.00246.x [doi]
PST - ppublish
SO  - Vet Comp Oncol. 2011 Sep;9(3):183-95. doi: 10.1111/j.1476-5829.2010.00246.x. Epub 
      2010 Sep 30.