PMID- 21849708
OWN - NLM
STAT- MEDLINE
DCOM- 20120412
LR  - 20161125
IS  - 1529-7268 (Electronic)
IS  - 0006-3363 (Linking)
VI  - 85
IP  - 6
DP  - 2011 Dec
TI  - Role of angiotensin II in the periovulatory epidermal growth factor-like cascade 
      in bovine granulosa cells in vitro.
PG  - 1167-74
LID - 10.1095/biolreprod.111.094193 [doi]
AB  - Angiotensin II (AGT-2) induces follicular prostaglandin release in a number of 
      species and ovulation in rabbits. Conversely, AGT-2 antagonists block ovulation 
      in cattle. To determine the mechanism of action of AGT-2, we used a bovine 
      granulosa cell model in which luteinizing hormone (LH) increased the expression 
      of genes essential for ovulation in a time- and dose-dependent manner. The 
      addition of AGT-2 to LH-stimulated cells significantly increased abundance of 
      prostaglandin-endoperoxide synthase 2 (PTGS2) mRNA and protein, whereas AGT-2 
      alone had no effect. Upstream of PTGS2, AGT-2 increased abundance of mRNA 
      encoding the epidermal growth factor-like ligands amphiregulin (AREG) and 
      epiregulin (EREG) at 6 h posttreatment and abundance of a disintegrin and 
      metalloprotease 17 (ADAM17), a sheddase, within 3 h of treatment. Inhibiting 
      sheddase activity abolished the stimulatory effect of AGT-2 on AREG, EREG, and 
      PTGS2 mRNA. The addition of selective AGT-2 antagonists to cells stimulated with 
      LH plus AGT-2 demonstrated that AGT-2 did not act through the type 1 receptor and 
      did not increase mitogen-activated protein kinase 3/1 phosphorylation. Combined 
      with previous data from studies in vitro, we conclude that AGT-2 is an essential 
      cofactor for LH in the early increase of ADAM expression/activity that induces 
      the cascade of events leading to ovulation.
FAU - Portela, Valerio M
AU  - Portela VM
AD  - Centre de Recherche en Reproduction Animale, Universite de Montreal, 
      St-Hyacinthe, Quebec, Canada.
FAU - Zamberlam, Gustavo
AU  - Zamberlam G
FAU - Goncalves, Paulo B D
AU  - Goncalves PB
FAU - de Oliveira, Joao F C
AU  - de Oliveira JF
FAU - Price, Christopher A
AU  - Price CA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110817
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Amphiregulin)
RN  - 0 (Epiregulin)
RN  - 0 (Glycoproteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 11128-99-7 (Angiotensin II)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 3.4.24.- (ADAM Proteins)
RN  - EC 3.4.24.86 (ADAM17 Protein)
SB  - IM
MH  - ADAM Proteins/metabolism
MH  - ADAM17 Protein
MH  - Amphiregulin
MH  - Angiotensin II/*physiology
MH  - Animals
MH  - Cattle
MH  - *Cell Culture Techniques
MH  - Cells, Cultured
MH  - Cyclooxygenase 2/metabolism
MH  - Epidermal Growth Factor/metabolism
MH  - Epiregulin
MH  - Female
MH  - Glycoproteins/metabolism
MH  - Granulosa Cells/*metabolism
MH  - Intercellular Signaling Peptides and Proteins/metabolism
MH  - Luteinizing Hormone/*physiology
MH  - *Ovulation
EDAT- 2011/08/19 06:00
MHDA- 2012/04/13 06:00
CRDT- 2011/08/19 06:00
PHST- 2011/08/19 06:00 [entrez]
PHST- 2011/08/19 06:00 [pubmed]
PHST- 2012/04/13 06:00 [medline]
AID - biolreprod.111.094193 [pii]
AID - 10.1095/biolreprod.111.094193 [doi]
PST - ppublish
SO  - Biol Reprod. 2011 Dec;85(6):1167-74. doi: 10.1095/biolreprod.111.094193. Epub 
      2011 Aug 17.