PMID- 21850157
OWN - NLM
STAT- MEDLINE
DCOM- 20111206
LR  - 20220408
IS  - 1090-0535 (Electronic)
IS  - 1090-0535 (Linking)
VI  - 17
DP  - 2011
TI  - High-mobility group box-1 and biomarkers of inflammation in the vitreous from 
      patients with proliferative diabetic retinopathy.
PG  - 1829-38
AB  - PURPOSE: To measure levels of high-mobility group box -1 (HMGB1) and soluble 
      receptor for advanced glycation end products (sRAGE) in the vitreous fluid from 
      patients with proliferative diabetic retinopathy (PDR) and to correlate their 
      levels with clinical disease activity and the levels of the inflammatory 
      biomarkers monocyte chemoattractant protein-1 (MCP-1), soluble intercellular 
      adhesion molecule-1 (sICAM-1), interleukin-1beta (IL-1beta), and granulocyte macrophage 
      colony-stimulating factor (GM-CSF). In addition, we examined the expression of 
      HMGB1 in the retinas of diabetic mice. METHODS: Vitreous samples from 29 PDR and 
      17 nondiabetic patients were studied by enzyme-linked immunosorbent assay. 
      Retinas of mice were examined by immunofluorescence analysis and western 
      blotting. RESULTS: HMGB1 was detected in all vitreous samples and sRAGE was 
      detected in 5 PDR samples. IL-1beta was detected in 3PDR samples and GM-CSF was not 
      detected. Mean HMGB1 levels in PDR with active neovascularization were twofold 
      and threefold higher than that in inactive PDR and nondiabetic patients, 
      respectively. Mean HMGB1 levels in PDR patients with hemorrhage were 
      significantly higher than those in PDR patients without hemorrhage and 
      nondiabetic patients (p=0.0111). There were significant correlations between 
      levels of HMGB1 and levels of MCP-1 (r=0.333, p=0.025) and sICAM-1 (r=0.548, 
      p<0.001). HMGB1 expression was also upregulated in the retinas of diabetic mice. 
      CONCLUSIONS: Subclinical chronic inflammation might contribute to the progression 
      of PDR.
FAU - El-Asrar, Ahmed M Abu
AU  - El-Asrar AM
AD  - Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, 
      Saudi Arabia. abuasrar@KSU.edu.sa
FAU - Nawaz, Mohd Imtiaz
AU  - Nawaz MI
FAU - Kangave, Dustan
AU  - Kangave D
FAU - Geboes, Karel
AU  - Geboes K
FAU - Ola, Mohammad Shamsul
AU  - Ola MS
FAU - Ahmad, Saif
AU  - Ahmad S
FAU - Al-Shabrawey, Mohamed
AU  - Al-Shabrawey M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110706
PL  - United States
TA  - Mol Vis
JT  - Molecular vision
JID - 9605351
RN  - 0 (Biomarkers)
RN  - 0 (Chemokine CCL2)
RN  - 0 (HMGB1 Protein)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Receptor for Advanced Glycation End Products)
RN  - 0 (Receptors, Immunologic)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Biomarkers/*analysis
MH  - Blotting, Western
MH  - Chemokine CCL2/analysis/biosynthesis
MH  - Diabetes Complications/*metabolism/pathology/surgery
MH  - Diabetes Mellitus/*metabolism/pathology/surgery
MH  - Diabetic Retinopathy/etiology/*metabolism/pathology/surgery
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - HMGB1 Protein/analysis/biosynthesis
MH  - Humans
MH  - Inflammation/*metabolism
MH  - Intercellular Adhesion Molecule-1/analysis/biosynthesis
MH  - Interleukin-1beta/analysis/biosynthesis
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Middle Aged
MH  - Receptor for Advanced Glycation End Products
MH  - Receptors, Immunologic/analysis/biosynthesis
MH  - Retina/*metabolism/pathology
MH  - Retinal Neovascularization/etiology/*metabolism/pathology/surgery
MH  - Vitrectomy
MH  - Vitreoretinopathy, Proliferative/etiology/*metabolism/pathology/surgery
MH  - Vitreous Body/*metabolism/pathology/surgery
PMC - PMC3137555
EDAT- 2011/08/19 06:00
MHDA- 2011/12/13 00:00
PMCR- 2011/01/01
CRDT- 2011/08/19 06:00
PHST- 2011/03/09 00:00 [received]
PHST- 2011/07/01 00:00 [accepted]
PHST- 2011/08/19 06:00 [entrez]
PHST- 2011/08/19 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
PHST- 2011/01/01 00:00 [pmc-release]
AID - 200 [pii]
AID - 2011MOLVIS0125 [pii]
PST - ppublish
SO  - Mol Vis. 2011;17:1829-38. Epub 2011 Jul 6.