PMID- 21851269
OWN - NLM
STAT- MEDLINE
DCOM- 20120326
LR  - 20211020
IS  - 1937-335X (Electronic)
IS  - 1937-3341 (Print)
IS  - 1937-3341 (Linking)
VI  - 17
IP  - 23-24
DP  - 2011 Dec
TI  - Collagen-calcium phosphate cement scaffolds seeded with umbilical cord stem cells 
      for bone tissue engineering.
PG  - 2943-54
LID - 10.1089/ten.tea.2010.0674 [doi]
AB  - Human umbilical cord mesenchymal stem cells (hUCMSCs) avoid the invasive 
      procedure required to harvest bone marrow MSCs. The addition of collagen fibers 
      into self-setting calcium phosphate cement (CPC) may increase the scaffold 
      strength, and enhance cell attachment and differentiation. The objectives of this 
      study were to develop a novel class of collagen-CPC composite scaffolds, and to 
      investigate hUCMSC attachment, proliferation, and osteogenic differentiation on 
      collagen-CPC scaffolds for the first time. Collagen fibers in CPC improved the 
      load-bearing capability. Flow cytometry showed that the hUCMSCs expressed cell 
      surface markers characteristic of MSCs, and were negative for hematopoietic and 
      endothelial cell markers. hUCMSCs proliferated rapidly in all CPC composite 
      scaffolds, with cell number increasing by sevenfold in 8 days. Cellular function 
      was enhanced with collagen fibers in CPC scaffolds. Cell density increased from 
      (645+/-60) cells/mm(2) on CPC with 0% collagen, to (1056+/-65) cells/mm(2) on CPC 
      with 8% collagen (p<0.05). The actin stress fibers inside the hUCMSCs were 
      stained, and the fluorescence intensity was doubled when the collagen in CPC was 
      increased by 0% to 8%. RT-PCR showed that hUCMSCs on CPC with collagen had higher 
      osteogenic expression than those on CPC without collagen. Alizarin Red S staining 
      revealed a great increase in mineralization by hUCMSCs on CPC with collagen than 
      that without collagen. In conclusion, hUCMSCs showed excellent proliferation, 
      differentiation, and synthesis of bone minerals in collagen-CPC composite 
      scaffolds for the first time. The novel hUCMSC-seeded collagen-CPC construct with 
      superior cell function and load-bearing capability is promising to enhance bone 
      regeneration in a wide range of orthopedic and craniofacial applications.
FAU - Thein-Han, WahWah
AU  - Thein-Han W
AD  - Biomaterials and Tissue Engineering Division, Department of Endodontics, 
      Prosthodontics and Operative Dentistry, University of Maryland Dental School, 
      Baltimore, MD 21201, USA.
FAU - Xu, Hockin H K
AU  - Xu HH
LA  - eng
GR  - R01 DE014190/DE/NIDCR NIH HHS/United States
GR  - R01 DE14190/DE/NIDCR NIH HHS/United States
GR  - R01 DE17974/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110818
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
RN  - 0 (Actins)
RN  - 0 (Anthraquinones)
RN  - 0 (Bone Cements)
RN  - 0 (Calcium Phosphates)
RN  - 60MEW57T9G (alizarin)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - Anthraquinones/metabolism
MH  - Bone Cements/*pharmacology
MH  - Bone and Bones/*drug effects
MH  - Calcium Phosphates/*pharmacology
MH  - Cattle
MH  - Cell Adhesion/drug effects
MH  - Cell Count
MH  - Cell Differentiation/drug effects/genetics
MH  - Cell Survival/drug effects
MH  - Collagen/*pharmacology
MH  - Fluorescence
MH  - Humans
MH  - Immunophenotyping
MH  - Mechanical Phenomena/drug effects
MH  - Mesenchymal Stem Cells/*cytology/drug effects/metabolism
MH  - Microspheres
MH  - Osteogenesis/drug effects/genetics
MH  - Stress Fibers/metabolism
MH  - Tissue Engineering/*methods
MH  - Tissue Scaffolds/*chemistry
MH  - Umbilical Cord/cytology
PMC - PMC3226062
EDAT- 2011/08/20 06:00
MHDA- 2012/03/27 06:00
PMCR- 2012/12/01
CRDT- 2011/08/20 06:00
PHST- 2011/08/20 06:00 [entrez]
PHST- 2011/08/20 06:00 [pubmed]
PHST- 2012/03/27 06:00 [medline]
PHST- 2012/12/01 00:00 [pmc-release]
AID - 10.1089/ten.tea.2010.0674 [pii]
AID - 10.1089/ten.tea.2010.0674 [doi]
PST - ppublish
SO  - Tissue Eng Part A. 2011 Dec;17(23-24):2943-54. doi: 10.1089/ten.tea.2010.0674. 
      Epub 2011 Aug 18.