PMID- 21852056
OWN - NLM
STAT- MEDLINE
DCOM- 20120118
LR  - 20110919
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 56
IP  - 5
DP  - 2011 Dec 15
TI  - Quantification of unfractionated heparin in human plasma and whole blood by means 
      of novel fluorogenic anti-FXa assays.
PG  - 992-7
LID - 10.1016/j.jpba.2011.07.025 [doi]
AB  - Novel and sensitive plate-based fluorogenic anti-factor Xa (FXa) assays were 
      investigated to quantify unfractionated heparin (UFH) in human plasma and whole 
      blood within the therapeutic ranges of 0-1.6 U/mL and 0-0.8 U/mL, respectively. 
      Two fluorogenic anti-FXa assay methods were defined for low (0-0.6 U/mL) and high 
      (0.6-1.2 U/mL) pharmacologically relevant UFH concentration ranges in pooled 
      human plasma. In both cases significant differences were observed at intervals of 
      0.2 U/mL (P<0.05). The semi-logarithmic plots of the calibration curves in the 
      low and high UFH range were both fitted to linear regressions with correlation 
      coefficients of 0.96 and >0.99, respectively. The assay was also optimized for 
      whole blood which was capable of differentiating UFH concentrations at intervals 
      of 0.2 U/mL (P<0.05) in the range of 0-0.4 U/mL. The statistically different 
      results were fitted to a linear regression with a correlation coefficient of 
      >0.99. The results obtained in this study could assist diagnostic laboratories 
      towards improved monitoring of UFH therapy.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Castro-Lopez, Vanessa
AU  - Castro-Lopez V
AD  - Biomedical Diagnostics Institute, National Centre for Sensor Research, Dublin 
      City University, Dublin 9, Ireland. vanessa.castro.lopez@gmail.com
FAU - Harris, Leanne F
AU  - Harris LF
FAU - O'Donnell, James S
AU  - O'Donnell JS
FAU - Killard, Anthony J
AU  - Killard AJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110728
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 9005-49-6 (Heparin)
RN  - EC 3.4.21.6 (Factor Xa)
SB  - IM
MH  - Adult
MH  - Calibration
MH  - Factor Xa/*analysis
MH  - Female
MH  - Heparin/*blood
MH  - Humans
MH  - Male
MH  - Spectrophotometry, Ultraviolet
EDAT- 2011/08/20 06:00
MHDA- 2012/01/19 06:00
CRDT- 2011/08/20 06:00
PHST- 2011/04/03 00:00 [received]
PHST- 2011/07/20 00:00 [revised]
PHST- 2011/07/21 00:00 [accepted]
PHST- 2011/08/20 06:00 [entrez]
PHST- 2011/08/20 06:00 [pubmed]
PHST- 2012/01/19 06:00 [medline]
AID - S0731-7085(11)00397-9 [pii]
AID - 10.1016/j.jpba.2011.07.025 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2011 Dec 15;56(5):992-7. doi: 10.1016/j.jpba.2011.07.025. 
      Epub 2011 Jul 28.