PMID- 21852101
OWN - NLM
STAT- MEDLINE
DCOM- 20120123
LR  - 20161125
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 28
IP  - 1
DP  - 2011 Oct 15
TI  - A new biosensor for glucose determination in serum based on up-converting 
      fluorescence resonance energy transfer.
PG  - 414-20
LID - 10.1016/j.bios.2011.07.057 [doi]
AB  - In this work, a new glucose sensor based on up-converting fluorescence resonance 
      energy transfer (UC-FRET) was developed. Up-converting phosphors (UCPs, NaYF(4): 
      Yb, Er), which were covalently labeled with Concanavalin A (ConA), were used as 
      the energy donor with thiolated beta-cyclodextrins (SH-beta-CDs) functionalized gold 
      nanoparticles as the energy acceptor. Due to the combination between ConA and 
      SH-beta-CDs, the energy donor and the acceptor were brought to close proximity, 
      resulting in the quenching of the fluorescence of UCPs by gold nanoparticles. In 
      the presence of glucose which competed with SH-beta-CDs towards the binding sites of 
      ConA, the biosensor (UCPs-ConA-SH-beta-CDs-Au) was decomposed and the energy donor 
      was separated from the acceptor. Therefore, the fluorescence of UCPs was restored 
      dependent on the concentration of glucose. The increase of UCPs fluorescence 
      intensity was proportional to glucose concentration within the range from 0.4 muM 
      to 10muM in aqueous buffer, with a limit of detection (LOD) of 0.043 muM. A same 
      linear range of glucose concentration was obtained in a human serum matrix (which 
      was pretreated and thus contained no glucose) with a slightly higher LOD (0.065 
      muM). The glucose sensor was applied to real human serum samples with the results 
      consistent with that of a classic hexokinase (HK) method, indicating that the 
      UC-FRET biosensor was competent for directly sensing glucose in serum samples 
      without optical interference, which benefited from the near infrared (NIR) 
      excitation nature of UCPs. The results of this work suggested that the UC-FRET 
      technique could be a promising alternative for detecting biomolecules in complex 
      biological sample matrixes for diagnostic purposes.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Peng, Jianhong
AU  - Peng J
AD  - Department of Laboratory, Zhongnan Hospital, Wuhan University School of Medicine, 
      Wuhan 430071, China.
FAU - Wang, Yuhui
AU  - Wang Y
FAU - Wang, Jialan
AU  - Wang J
FAU - Zhou, Xin
AU  - Zhou X
FAU - Liu, Zhihong
AU  - Liu Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110730
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Acrylic Resins)
RN  - 0 (Blood Glucose)
RN  - 11028-71-0 (Concanavalin A)
RN  - 4Q93RCW27E (carbopol 940)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Acrylic Resins/chemistry
MH  - Biosensing Techniques/*methods
MH  - Blood Glucose/*analysis
MH  - Concanavalin A/chemistry
MH  - Fluorescence Resonance Energy Transfer/*methods
MH  - Gold/chemistry
MH  - Humans
MH  - Nanoparticles/chemistry
EDAT- 2011/08/20 06:00
MHDA- 2012/01/24 06:00
CRDT- 2011/08/20 06:00
PHST- 2011/04/07 00:00 [received]
PHST- 2011/07/22 00:00 [revised]
PHST- 2011/07/22 00:00 [accepted]
PHST- 2011/08/20 06:00 [entrez]
PHST- 2011/08/20 06:00 [pubmed]
PHST- 2012/01/24 06:00 [medline]
AID - S0956-5663(11)00504-5 [pii]
AID - 10.1016/j.bios.2011.07.057 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2011 Oct 15;28(1):414-20. doi: 10.1016/j.bios.2011.07.057. 
      Epub 2011 Jul 30.