PMID- 2185257
OWN - NLM
STAT- MEDLINE
DCOM- 19900604
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 28
IP  - 4
DP  - 1990 Apr
TI  - Characterization of antibody reactivity to human T-cell lymphotropic virus types 
      I and II using immunoblot and radioimmunoprecipitation assays.
PG  - 646-50
AB  - We have characterized the immunoreactivity to human T-cell lymphotropic virus 
      type I (HTLV-I) among 26,983 persons of various seroprevalence groups by using 
      enzyme immunoassay, immunoblot (IB), and radioimmunoprecipitation assays (RIPA) 
      in accordance with Public Health Service recommended guidelines for the 
      interpretation of serologic test results for HTLV-I infection. IB-indeterminate 
      serum specimens (n = 178) were reactive to HTLV-I gag proteins, and no serum 
      contained only env reactivity. Overall, RIPA resolved 40% of IB-indeterminate 
      serum samples; however, the probability that RIPA would confirm IB-indeterminate 
      samples depended on the seroprevalence of the population tested. HTLV-I gag 
      p19-only reactivity on IB was not a reliable marker of HTLV-I infection, while 
      gag p24 reactivity on IB was clearly associated with positive seroreactive 
      specimens. IB and RIPA tests did not clearly distinguish between HTLV-I and 
      HTLV-II seroreactivities. These data emphasize that patterns of immunoreactivity 
      to HTLV-I antigens are dependent upon the seroprevalence of the risk groups 
      tested. In addition, RIPA detected antibodies to env proteins present in low 
      titer in a substantial number of IB gag-only reactive sera and resolved the 
      HTLV-I antibody status of these sera.
FAU - Hartley, T M
AU  - Hartley TM
AD  - Retrovirus Diseases Branch, Centers for Disease Control, Atlanta, Georgia 30333.
FAU - Khabbaz, R F
AU  - Khabbaz RF
FAU - Cannon, R O
AU  - Cannon RO
FAU - Kaplan, J E
AU  - Kaplan JE
FAU - Lairmore, M D
AU  - Lairmore MD
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Gene Products, gag)
RN  - 0 (HTLV-I Antibodies)
RN  - 0 (HTLV-II Antibodies)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
EIN - J Clin Microbiol 1990 Jun;28(6):1491
MH  - Gene Products, gag/immunology
MH  - HTLV-I Antibodies/*analysis
MH  - HTLV-I Infections/*immunology
MH  - HTLV-II Antibodies/*analysis
MH  - HTLV-II Infections/*immunology
MH  - Humans
MH  - Immunoblotting
MH  - Immunoenzyme Techniques
MH  - Precipitin Tests
MH  - Viral Envelope Proteins/immunology
PMC - PMC267769
EDAT- 1990/04/01 00:00
MHDA- 1990/04/01 00:01
PMCR- 1990/04/01
CRDT- 1990/04/01 00:00
PHST- 1990/04/01 00:00 [pubmed]
PHST- 1990/04/01 00:01 [medline]
PHST- 1990/04/01 00:00 [entrez]
PHST- 1990/04/01 00:00 [pmc-release]
AID - 10.1128/jcm.28.4.646-650.1990 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1990 Apr;28(4):646-50. doi: 10.1128/jcm.28.4.646-650.1990.