PMID- 21852897
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211020
IS  - 1178-6264 (Print)
IS  - 1178-6264 (Electronic)
IS  - 1178-6264 (Linking)
VI  - 2010
IP  - 3
DP  - 2010 Sep 1
TI  - Morphological Changes and Immunohistochemical Expression of RAGE and its Ligands 
      in the Sciatic Nerve of Hyperglycemic Pig (Sus Scrofa).
PG  - 47-59
AB  - The aim of our project was to study the effect of streptozotocin (STZ)-induced 
      hyperglycemia on sciatic nerve morphology, blood plasma markers and 
      immunohistochemical expression of RAGE (the Receptor for Advanced Glycation 
      End-products), and its ligands-S100B and Carboxymethyl Lysine (CML)-advanced 
      glycation endproduct (AGE) in the laboratory pig. Six months after 
      STZ-injections, blood plasma measurements, morphometric analysis of sciatic nerve 
      fiber density, immunofluorescent distribution of potential molecular neuropathy 
      contributors, ELISA measurement of plasma AGE level and HPLC analysis of sciatic 
      nerve levels of one of the pre-AGE and the glycolysis intermediate 
      products-methyl-glyoxal (MG) were performed. The results of our study revealed 
      that STZ-injected animals displayed elevated levels of plasma glucose, gamma 
      glutamyl transferase (GGT) and triglycerides. The sciatic nerve of STZ-injected 
      pigs revealed significantly lower numbers of small-diameter myelinated fibers, 
      higher immunoreactivity for RAGE and S100B and increased levels of MG as compared 
      to control animals. Our results correspond to clinical findings in human patients 
      with hyperglycemia/diabetes-evoked peripheral neuropathy and suggest that the 
      domestic pig may be a suitable large animal model for the study of mechanisms 
      underlying hyperglycemia-induced neurological complications in the peripheral 
      nerve and may serve as a relevant model for the pre-clinical assessment of 
      candidate drugs in neuropathy.
FAU - Juranek, Judyta K
AU  - Juranek JK
AD  - Division of Surgical Science, Columbia University Medical Center, New York, NY, 
      10032, USA.
FAU - Aleshin, Alexey
AU  - Aleshin A
FAU - Rattigan, Eileen M
AU  - Rattigan EM
FAU - Johnson, Lynne
AU  - Johnson L
FAU - Qu, Wu
AU  - Qu W
FAU - Song, Fei
AU  - Song F
FAU - Ananthakrishnan, Radha
AU  - Ananthakrishnan R
FAU - Quadri, Nosirudeen
AU  - Quadri N
FAU - Yan, Shi Du
AU  - Yan SD
FAU - Ramasamy, Ravichandran
AU  - Ramasamy R
FAU - Schmidt, Ann Marie
AU  - Schmidt AM
FAU - Geddis, Matthew S
AU  - Geddis MS
LA  - eng
GR  - P01 AG017490/AG/NIA NIH HHS/United States
GR  - P01 AG017490-090002/AG/NIA NIH HHS/United States
GR  - P01 AG026467/AG/NIA NIH HHS/United States
GR  - P01 AG026467-03/AG/NIA NIH HHS/United States
PT  - Journal Article
PL  - England
TA  - Biochem Insights
JT  - Biochemistry insights
JID - 101549953
PMC - PMC3156451
MID - NIHMS247418
COIS- Disclosures This manuscript has been read and approved by all authors. This paper 
      is unique and not under consideration by any other publication and has not been 
      published elsewhere. The authors and peer reviewers report no conflicts of 
      interest. The authors confirm that they have permission to reproduce any 
      copyrighted material.
EDAT- 2011/08/20 06:00
MHDA- 2011/08/20 06:01
PMCR- 2011/08/16
CRDT- 2011/08/20 06:00
PHST- 2011/08/20 06:00 [entrez]
PHST- 2011/08/20 06:00 [pubmed]
PHST- 2011/08/20 06:01 [medline]
PHST- 2011/08/16 00:00 [pmc-release]
AID - 10.4137/BCI.S5340 [doi]
PST - ppublish
SO  - Biochem Insights. 2010 Sep 1;2010(3):47-59. doi: 10.4137/BCI.S5340.