PMID- 21854616
OWN - NLM
STAT- MEDLINE
DCOM- 20111110
LR  - 20211020
IS  - 1471-2156 (Electronic)
IS  - 1471-2156 (Linking)
VI  - 12
DP  - 2011 Aug 19
TI  - A second-generation anchored genetic linkage map of the tammar wallaby (Macropus 
      eugenii).
PG  - 72
LID - 10.1186/1471-2156-12-72 [doi]
AB  - BACKGROUND: The tammar wallaby, Macropus eugenii, a small kangaroo used for 
      decades for studies of reproduction and metabolism, is the model Australian 
      marsupial for genome sequencing and genetic investigations. The production of a 
      more comprehensive cytogenetically-anchored genetic linkage map will 
      significantly contribute to the deciphering of the tammar wallaby genome. It has 
      great value as a resource to identify novel genes and for comparative studies, 
      and is vital for the ongoing genome sequence assembly and gene ordering in this 
      species. RESULTS: A second-generation anchored tammar wallaby genetic linkage map 
      has been constructed based on a total of 148 loci. The linkage map contains the 
      original 64 loci included in the first-generation map, plus an additional 84 
      microsatellite loci that were chosen specifically to increase coverage and assist 
      with the anchoring and orientation of linkage groups to chromosomes. These 
      additional loci were derived from (a) sequenced BAC clones that had been 
      previously mapped to tammar wallaby chromosomes by fluorescence in situ 
      hybridization (FISH), (b) End sequence from BACs subsequently FISH-mapped to 
      tammar wallaby chromosomes, and (c) tammar wallaby genes orthologous to opossum 
      genes predicted to fill gaps in the tammar wallaby linkage map as well as three 
      X-linked markers from a published study. Based on these 148 loci, eight linkage 
      groups were formed. These linkage groups were assigned (via FISH-mapped markers) 
      to all seven autosomes and the X chromosome. The sex-pooled map size is 1402.4 
      cM, which is estimated to provide 82.6% total coverage of the genome, with an 
      average interval distance of 10.9 cM between adjacent markers. The overall ratio 
      of female/male map length is 0.84, which is comparable to the ratio of 0.78 
      obtained for the first-generation map. CONCLUSIONS: Construction of this 
      second-generation genetic linkage map is a significant step towards complete 
      coverage of the tammar wallaby genome and considerably extends that of the 
      first-generation map. It will be a valuable resource for ongoing tammar wallaby 
      genetic research and assembling the genome sequence. The sex-pooled map is 
      available online at http://compldb.angis.org.au/.
FAU - Wang, Chenwei
AU  - Wang C
AD  - Reprogen, Faculty of Veterinary Science, The University of Sydney, Sydney, NSW 
      2006, Australia. cwan0629@uni.sydney.edu.au
FAU - Webley, Lee
AU  - Webley L
FAU - Wei, Ke-jun
AU  - Wei KJ
FAU - Wakefield, Matthew J
AU  - Wakefield MJ
FAU - Patel, Hardip R
AU  - Patel HR
FAU - Deakin, Janine E
AU  - Deakin JE
FAU - Alsop, Amber
AU  - Alsop A
FAU - Marshall Graves, Jennifer A
AU  - Marshall Graves JA
FAU - Cooper, Desmond W
AU  - Cooper DW
FAU - Nicholas, Frank W
AU  - Nicholas FW
FAU - Zenger, Kyall R
AU  - Zenger KR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110819
PL  - England
TA  - BMC Genet
JT  - BMC genetics
JID - 100966978
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Animals
MH  - *Chromosome Mapping
MH  - Chromosomes, Artificial, Bacterial
MH  - Female
MH  - Genetic Markers
MH  - Genotype
MH  - Macropodidae/*genetics
MH  - Male
PMC - PMC3176194
EDAT- 2011/08/23 06:00
MHDA- 2011/11/11 06:00
PMCR- 2011/08/19
CRDT- 2011/08/23 06:00
PHST- 2010/09/09 00:00 [received]
PHST- 2011/08/19 00:00 [accepted]
PHST- 2011/08/23 06:00 [entrez]
PHST- 2011/08/23 06:00 [pubmed]
PHST- 2011/11/11 06:00 [medline]
PHST- 2011/08/19 00:00 [pmc-release]
AID - 1471-2156-12-72 [pii]
AID - 10.1186/1471-2156-12-72 [doi]
PST - epublish
SO  - BMC Genet. 2011 Aug 19;12:72. doi: 10.1186/1471-2156-12-72.