PMID- 21854760
OWN - NLM
STAT- MEDLINE
DCOM- 20120118
LR  - 20231213
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 415
IP  - 2
DP  - 2011 Nov 18
TI  - Transcriptional activity of DNMT3B in pancreatic cancer cells: effects of -149 
      (C-->T) promoter polymorphism.
PG  - 220-3
LID - 10.1016/j.bbrc.2011.07.115 [doi]
AB  - Polymorphic C-to-T change in the promoter region of DNA-methyltransferase-3B 
      (DNMT3B) gene is associated with risk of several cancers. The aim of this study 
      was to investigate the effect of DNMT3B promoter genetic variant on its 
      transcriptional activity and to compare activity in several pancreatic cell 
      lines. DNMT3B promoter constructs carrying either -149C allele or -149T allele 
      were transiently transfected into pancreatic cancer cells. In promoter assaying, 
      carriage of -149T allele showed only a slight activity (1.1-fold) in Mia cells 
      (p=0.462). In contrast, significant increase (3.8-fold) in activity of -149T 
      allele was shown in SU86.86 pancreatic cancer cells (p=0.0001). These preliminary 
      findings suggest that genetic variance may influence DNMT3B expression in 
      pancreatic cancer. Further studies are needed.
CI  - Published by Elsevier Inc.
FAU - Xiao, Yongmei
AU  - Xiao Y
AD  - Office of Regulatory Activities, National Center for Toxicological Research, 
      Jefferson, AR 72079, United States.
FAU - Word, Beverly
AU  - Word B
FAU - Hammons, George
AU  - Hammons G
FAU - Lyn-Cook, Beverly
AU  - Lyn-Cook B
LA  - eng
PT  - Journal Article
DEP - 20110811
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferases)
SB  - IM
MH  - Cell Line, Tumor
MH  - DNA (Cytosine-5-)-Methyltransferases/*genetics
MH  - DNA Methylation
MH  - *Gene Expression Regulation, Enzymologic
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Pancreatic Neoplasms/*genetics
MH  - Promoter Regions, Genetic/genetics
MH  - Transcription, Genetic
MH  - DNA Methyltransferase 3B
EDAT- 2011/08/23 06:00
MHDA- 2012/01/19 06:00
CRDT- 2011/08/23 06:00
PHST- 2011/07/05 00:00 [received]
PHST- 2011/07/29 00:00 [accepted]
PHST- 2011/08/23 06:00 [entrez]
PHST- 2011/08/23 06:00 [pubmed]
PHST- 2012/01/19 06:00 [medline]
AID - S0006-291X(11)01359-3 [pii]
AID - 10.1016/j.bbrc.2011.07.115 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2011 Nov 18;415(2):220-3. doi: 
      10.1016/j.bbrc.2011.07.115. Epub 2011 Aug 11.