PMID- 21854779
OWN - NLM
STAT- MEDLINE
DCOM- 20111219
LR  - 20110905
IS  - 1879-0135 (Electronic)
IS  - 0020-7519 (Linking)
VI  - 41
IP  - 11
DP  - 2011 Sep
TI  - Characterization of TcHMGB, a high mobility group B family member protein from 
      Trypanosoma cruzi.
PG  - 1149-56
LID - 10.1016/j.ijpara.2011.06.009 [doi]
AB  - High mobility group B (HMGB) proteins are highly abundant non-histone chromatin 
      proteins that play important roles in the execution and control of many nuclear 
      functions. Based on homology searches, we identified the coding sequence for the 
      TcHMGB protein, an HMGB family member from Trypanosoma cruzi. TcHMGB has two HMG 
      box domains, similar to mammalian HMGBs, but lacks the typical C-terminal acidic 
      tail. Instead, it contains a 110 amino acid long N-terminal domain. The TcHMGB 
      N-terminal domain is conserved between the TriTryp sequences (70-80% similarity) 
      and seems to be characteristic of kinetoplastid HMGBs. Despite these differences, 
      TcHMGB maintains HMG box architectural functions: we demonstrated that the 
      trypanosomatid HMGB binds distorted DNA structures such as cruciform DNA in gel 
      shift assays. TcHMGB is also able to bend linear DNA as determined by T4 ligase 
      circularization assays, similar to other HMGB family members. Immunofluorescence 
      and western blot assays showed that TcHMGB is a nuclear protein expressed in all 
      life cycle stages. Protein levels, however, seem to vary throughout the life 
      cycle, which may be related to previously described changes in heterochromatin 
      distribution and transcription rates.
CI  - Copyright (c) 2011 Australian Society for Parasitology Inc. Published by Elsevier 
      Ltd. All rights reserved.
FAU - Cribb, Pamela
AU  - Cribb P
AD  - Instituto de Biologia Molecular y Celular de Rosario (IBR-CONICET), Facultad de 
      Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Suipacha 
      531, Rosario CP2000, Argentina.
FAU - Perozzi, Marina
AU  - Perozzi M
FAU - Villanova, Gabriela Vanina
AU  - Villanova GV
FAU - Trochine, Andrea
AU  - Trochine A
FAU - Serra, Esteban
AU  - Serra E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110805
PL  - England
TA  - Int J Parasitol
JT  - International journal for parasitology
JID - 0314024
RN  - 0 (High Mobility Group Proteins)
RN  - 0 (Protozoan Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Gene Expression Regulation, Developmental
MH  - High Mobility Group Proteins/chemistry/genetics/*metabolism
MH  - Molecular Sequence Data
MH  - Multigene Family
MH  - Protein Binding
MH  - Protein Structure, Tertiary
MH  - Protein Transport
MH  - Protozoan Proteins/chemistry/genetics/*metabolism
MH  - Sequence Alignment
MH  - Trypanosoma cruzi/chemistry/genetics/growth & development/*metabolism
EDAT- 2011/08/23 06:00
MHDA- 2011/12/20 06:00
CRDT- 2011/08/23 06:00
PHST- 2010/11/09 00:00 [received]
PHST- 2011/06/03 00:00 [revised]
PHST- 2011/06/25 00:00 [accepted]
PHST- 2011/08/23 06:00 [entrez]
PHST- 2011/08/23 06:00 [pubmed]
PHST- 2011/12/20 06:00 [medline]
AID - S0020-7519(11)00179-2 [pii]
AID - 10.1016/j.ijpara.2011.06.009 [doi]
PST - ppublish
SO  - Int J Parasitol. 2011 Sep;41(11):1149-56. doi: 10.1016/j.ijpara.2011.06.009. Epub 
      2011 Aug 5.