PMID- 21855541
OWN - NLM
STAT- MEDLINE
DCOM- 20111219
LR  - 20110913
IS  - 1090-2422 (Electronic)
IS  - 0014-4827 (Linking)
VI  - 317
IP  - 17
DP  - 2011 Oct 15
TI  - N-cadherin mediates angiogenesis by regulating monocyte chemoattractant protein-1 
      expression via PI3K/Akt signaling in prostate cancer cells.
PG  - 2512-21
LID - 10.1016/j.yexcr.2011.07.024 [doi]
AB  - Over the past decade, evidence continues to mount showing that N-cadherin is a 
      critical protein in cancer progression and metastasis. In the present study, we 
      evaluated the expression of N-cadherin in human prostate cancer tissue specimens 
      and cell lines. Enhanced expression of N-cadherin was observed in both the 
      malignant and bone-metastasized prostate tissue specimens compared to the healthy 
      prostate tissues. Consistent with the tissue array data, N-cadherin was highly 
      expressed in PC3, but not in Du145 and LNCaP human prostate cell lines. Based on 
      cell to cell binding assay, we found that N-cadherin expression facilitates 
      homotypic interaction between human prostate cancer cells and human microvascular 
      endothelial cells (HMEC). Human angiogenesis antibody array and in vitro 
      angiogenesis assay showed that siRNA-mediated knockdown of N-cadherin reduced the 
      secretion of monocyte chemoattractant protein-1 (MCP-1), which played a potential 
      role in stimulating capillary network formation of HMEC. Additionally, culture 
      supernatant of Du145 cells transfected with full-length N-cadherin expressing 
      plasmid showed increased MCP-1 expression and chemoattractant ability compared to 
      normal Du145 cells. Further, we noticed that blocking PI3K activity inhibited 
      N-cadherin mediated MCP-1 expression. Our data demonstrated that N-cadherin in 
      prostate cancer cell mediates cell-cell adhesion and regulates MCP-1 expression 
      via the PI3K/Akt signaling pathway.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Nalla, Arun Kumar
AU  - Nalla AK
AD  - Department of Cancer Biology and Pharmacology, University of Illinois College of 
      Medicine at Peoria, Peoria, IL, USA.
FAU - Estes, Norman
AU  - Estes N
FAU - Patel, Jitendra
AU  - Patel J
FAU - Rao, Jasti S
AU  - Rao JS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110809
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Antigens, CD)
RN  - 0 (CDH2 protein, human)
RN  - 0 (Cadherins)
RN  - 0 (Chemokine CCL2)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Antigens, CD/biosynthesis/genetics/*metabolism
MH  - Cadherins/biosynthesis/genetics/*metabolism
MH  - Cell Adhesion
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis/metabolism
MH  - Humans
MH  - Male
MH  - Neovascularization, Pathologic/*metabolism
MH  - Phosphatidylinositol 3-Kinases/*metabolism
MH  - Prostatic Neoplasms/*metabolism/pathology
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - *Signal Transduction
EDAT- 2011/08/23 06:00
MHDA- 2011/12/20 06:00
CRDT- 2011/08/23 06:00
PHST- 2011/05/25 00:00 [received]
PHST- 2011/07/27 00:00 [revised]
PHST- 2011/07/29 00:00 [accepted]
PHST- 2011/08/23 06:00 [entrez]
PHST- 2011/08/23 06:00 [pubmed]
PHST- 2011/12/20 06:00 [medline]
AID - S0014-4827(11)00310-7 [pii]
AID - 10.1016/j.yexcr.2011.07.024 [doi]
PST - ppublish
SO  - Exp Cell Res. 2011 Oct 15;317(17):2512-21. doi: 10.1016/j.yexcr.2011.07.024. Epub 
      2011 Aug 9.