PMID- 21855955
OWN - NLM
STAT- MEDLINE
DCOM- 20111118
LR  - 20110905
IS  - 1879-2448 (Electronic)
IS  - 0043-1354 (Linking)
VI  - 45
IP  - 16
DP  - 2011 Oct 15
TI  - Methanogen community structure-activity relationship and bioaugmentation of 
      overloaded anaerobic digesters.
PG  - 5249-56
LID - 10.1016/j.watres.2011.07.035 [doi]
AB  - Accumulation of acids in anaerobic digesters after organic overload can inhibit 
      or stop CH4 production. Therefore, methods to reduce acid concentrations would be 
      helpful. One potential method to improve recovery involves bioaugmentation, 
      addition of specific microorganisms to improve performance. In this study, 
      transiently overloaded digesters were bioaugmented with a propionate-degrading 
      enrichment culture in an effort to decrease recovery time. Biomass samples from 
      14 different, full-scale anaerobic digesters were screened for specific 
      methanogenic activity (SMA) against propionate; the microbial communities were 
      also compared. SMA values spanned two orders of magnitude. Principal component 
      analysis of denaturing gradient gel electrophoresis (DGGE) banding patterns for a 
      functional gene (mcrA) suggested an underlying community structure-activity 
      relationship; the presence of hydrogenotrophic methanogens closely related to 
      Methanospirillum hungatei and Methanobacterium beijingense was associated with 
      high propionate SMA values. The biomass sample demonstrating the highest SMA was 
      enriched for propionate degrading activity and then used to bioaugment overloaded 
      digesters. Bioaugmented digesters recovered more rapidly following the organic 
      overload, requiring approximately 25 days (2.5 solids retention times (SRTs)) 
      less to recover compared to non-bioaugmented digesters. Benefits of 
      bioaugmentation continued for more than 12 SRTs after organic overload. 
      Bioaugmentation is a promising approach to decrease recovery time after organic 
      overload.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Tale, V P
AU  - Tale VP
AD  - Department of Civil and Environmental Engineering, Marquette University, P.O. Box 
      1881, Milwaukee, WI 53201, United States. vpt262@gmail.com
FAU - Maki, J S
AU  - Maki JS
FAU - Struble, C A
AU  - Struble CA
FAU - Zitomer, D H
AU  - Zitomer DH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110731
PL  - England
TA  - Water Res
JT  - Water research
JID - 0105072
RN  - 0 (Culture Media)
RN  - 0 (DNA Primers)
SB  - IM
MH  - Anaerobiosis
MH  - Base Sequence
MH  - Biomass
MH  - *Bioreactors
MH  - Culture Media
MH  - DNA Primers
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Euryarchaeota/*isolation & purification/metabolism
EDAT- 2011/08/23 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/08/23 06:00
PHST- 2011/03/26 00:00 [received]
PHST- 2011/07/07 00:00 [revised]
PHST- 2011/07/25 00:00 [accepted]
PHST- 2011/08/23 06:00 [entrez]
PHST- 2011/08/23 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - S0043-1354(11)00432-5 [pii]
AID - 10.1016/j.watres.2011.07.035 [doi]
PST - ppublish
SO  - Water Res. 2011 Oct 15;45(16):5249-56. doi: 10.1016/j.watres.2011.07.035. Epub 
      2011 Jul 31.