PMID- 21856217
OWN - NLM
STAT- MEDLINE
DCOM- 20120131
LR  - 20220317
IS  - 1873-3557 (Electronic)
IS  - 1386-1425 (Linking)
VI  - 82
IP  - 1
DP  - 2011 Nov
TI  - Spectroscopic analysis on the resveratrol-DNA binding interactions at 
      physiological pH.
PG  - 213-6
LID - 10.1016/j.saa.2011.07.037 [doi]
AB  - The interaction of resveratrol with calf thymus deoxyribonucleic acid (ctDNA) 
      under physiological conditions (Tris-HCl buffer solutions, pH 7.4) was studied by 
      spectroscopy, fluorescence spectroscopy and viscosity measurement method, 
      respectively. Results indicated that a complex of resveratrol with ctDNA was 
      formed with a binding constant of K(17 degrees C) = 5.49x10(3) L mol(-1) and K(37 degrees C) = 
      1.90x10(4) L mol(-1). The fluorescence quenching mechanism of acridine orange 
      (AO)-ctDNA by resveratrol was shown to be a static quenching type. The 
      thermodynamic parameters of the complex were calculated by a double reciprocal 
      method: Delta(r)H(m)(s) = 4.64x10(4)  J  mol(-1), Delta(r)S(m)(s) = 231.8  J  K(-1) 
       mol(-1) and Delta(r)G(m)(s) = -2.54x10(4)  J  mol(-1) (37 degrees C). Spectroscopic 
      techniques together with viscosity determination provided evidences of 
      intercalation mode of binding for the interaction between resveratrol and ctDNA.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Zhang, Shufang
AU  - Zhang S
AD  - College of Chemistry and Chemical Engineering, Qufu Normal University, Shandong, 
      Qufu 273165, People's Republic of China. zhshf999@sina.com
FAU - Sun, Xuejun
AU  - Sun X
FAU - Jing, Zhihong
AU  - Jing Z
FAU - Qu, Fengli
AU  - Qu F
LA  - eng
PT  - Journal Article
DEP - 20110803
PL  - England
TA  - Spectrochim Acta A Mol Biomol Spectrosc
JT  - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
JID - 9602533
RN  - 0 (Stilbenes)
RN  - 9007-49-2 (DNA)
RN  - 91080-16-9 (calf thymus DNA)
RN  - Q369O8926L (Resveratrol)
SB  - IM
MH  - Absorption
MH  - Animals
MH  - Cattle
MH  - DNA/*chemistry
MH  - Equipment Design
MH  - Hydrogen-Ion Concentration
MH  - Kinetics
MH  - Models, Chemical
MH  - Protein Binding
MH  - Resveratrol
MH  - Spectrometry, Fluorescence/methods
MH  - Spectrophotometry/*methods
MH  - Spectrophotometry, Ultraviolet/methods
MH  - Stilbenes/*chemistry
MH  - Thermodynamics
MH  - Viscosity
EDAT- 2011/08/23 06:00
MHDA- 2012/02/01 06:00
CRDT- 2011/08/23 06:00
PHST- 2010/07/21 00:00 [received]
PHST- 2011/02/28 00:00 [revised]
PHST- 2011/07/13 00:00 [accepted]
PHST- 2011/08/23 06:00 [entrez]
PHST- 2011/08/23 06:00 [pubmed]
PHST- 2012/02/01 06:00 [medline]
AID - S1386-1425(11)00627-5 [pii]
AID - 10.1016/j.saa.2011.07.037 [doi]
PST - ppublish
SO  - Spectrochim Acta A Mol Biomol Spectrosc. 2011 Nov;82(1):213-6. doi: 
      10.1016/j.saa.2011.07.037. Epub 2011 Aug 3.