PMID- 21856253
OWN - NLM
STAT- MEDLINE
DCOM- 20111114
LR  - 20220405
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 879
IP  - 26
DP  - 2011 Sep 15
TI  - Metabolic and pharmacokinetic studies of curcumin, demethoxycurcumin and 
      bisdemethoxycurcumin in mice tumor after intragastric administration of 
      nanoparticle formulations by liquid chromatography coupled with tandem mass 
      spectrometry.
PG  - 2751-8
LID - 10.1016/j.jchromb.2011.07.042 [doi]
AB  - This paper aims to investigate the metabolism and pharmacokinetics of curcumin, 
      demethoxycurcumin and bisdemethoxycurcumin in mice tumor. To improve water 
      solubility, nanoparticle formulations were prepared as curcuminoids-loaded solid 
      lipid nanoparticles (curcuminoids-SLNs) and curcumin-loaded solid lipid 
      nanoparticles (curcumin-SLNs). After intragastric administration to tumor-bearing 
      ICR mice, the plasma and tumor samples were analyzed by liquid chromatography 
      with ion trap mass spectrometry. We discovered that curcuminoids were mainly 
      present as glucuronides in plasma, whereas in free form in tumor tissue. A 
      validated LC/MS/MS method was established to determine the three free 
      curcuminoids in tumor homogenate. Samples were separated on a Zorbax SB-C(18) 
      column, eluted with acetonitrile-water (containing 0.1% formic acid), and 
      detected by TSQ Quantum triple quadrupole mass spectrometer in selected reaction 
      monitoring mode. The method showed good linearity (r(2)=0.997-0.999) over wide 
      dynamic ranges (2-6000 ng/mL). Variations within- and between-batch never 
      exceeded 11.2% and 13.4%, respectively. The extraction recovery rates ranged from 
      78.3% to 87.7%. The pharmacokinetics of curcuminoids in mice tumor fit 
      two-compartment model and first order elimination. For curcumin-SLNs group, the 
      dosing of 250 mg/kg of curcumin resulted in AUC((0-48 h)) of 2285 ngh/mL and 
      C(max) of 209 ng/mL. For curcuminoids-SLNs group, the dosing equivalent to 138 
      mg/kg of curcumin resulted in higher tumor concentrations (AUC=2811 ngh/mL, 
      C(max)=285 ng/mL). It appeared that co-existing curcuminoids improved the 
      bioavailability of curcumin.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Li, Rui
AU  - Li R
AD  - State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical 
      Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China.
FAU - Qiao, Xue
AU  - Qiao X
FAU - Li, Qingyan
AU  - Li Q
FAU - He, Rong
AU  - He R
FAU - Ye, Min
AU  - Ye M
FAU - Xiang, Cheng
AU  - Xiang C
FAU - Lin, Xionghao
AU  - Lin X
FAU - Guo, Dean
AU  - Guo D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110806
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Biphenyl Compounds)
RN  - 0 (Diarylheptanoids)
RN  - 0 (Lignans)
RN  - 0 (Lipids)
RN  - 11513CCO0N (honokiol)
RN  - 2EFO1BP34R (bisdemethoxycurcumin)
RN  - IT942ZTH98 (Curcumin)
RN  - W2F8059T80 (demethoxycurcumin)
SB  - IM
MH  - Animals
MH  - Area Under Curve
MH  - Biphenyl Compounds/chemistry
MH  - Chromatography, Liquid/*methods
MH  - Curcumin/administration & dosage/*analogs & 
      derivatives/metabolism/pharmacokinetics
MH  - Diarylheptanoids
MH  - Drug Delivery Systems
MH  - Drug Stability
MH  - Lignans/chemistry
MH  - Linear Models
MH  - Lipids
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Nanoparticles/*administration & dosage/chemistry
MH  - Neoplasm Transplantation
MH  - Neoplasms/chemistry/*metabolism
MH  - Reproducibility of Results
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2011/08/23 06:00
MHDA- 2011/11/15 06:00
CRDT- 2011/08/23 06:00
PHST- 2011/05/16 00:00 [received]
PHST- 2011/07/23 00:00 [revised]
PHST- 2011/07/31 00:00 [accepted]
PHST- 2011/08/23 06:00 [entrez]
PHST- 2011/08/23 06:00 [pubmed]
PHST- 2011/11/15 06:00 [medline]
AID - S1570-0232(11)00510-1 [pii]
AID - 10.1016/j.jchromb.2011.07.042 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Sep 15;879(26):2751-8. doi: 
      10.1016/j.jchromb.2011.07.042. Epub 2011 Aug 6.