PMID- 21856381
OWN - NLM
STAT- MEDLINE
DCOM- 20120625
LR  - 20240320
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 194
DP  - 2011 Oct 27
TI  - Reduction of high-fat diet-induced obesity after chronic administration of 
      brain-derived neurotrophic factor in the hypothalamic ventromedial nucleus.
PG  - 36-52
LID - 10.1016/j.neuroscience.2011.07.079 [doi]
AB  - An acute injection of brain-derived neurotrophic factor (BDNF) in the 
      hypothalamic ventromedial nucleus (VMN) decreases body weight by reducing feeding 
      and increasing energy expenditure (EE) in animals on standard laboratory chow. 
      Animals have divergent responses to high-fat diet (HFD) exposure, with some 
      developing obesity and others remaining lean. In the current study, we tested the 
      hypothesis that BDNF in the VMN reduces HFD-induced obesity. Seventy-two 10-week 
      old rats were allowed HFD ad libitum for 8 weeks and then prepared with bilateral 
      VMN cannulae. Animals were then divided into tertiles based on their fat mass 
      rank: high, intermediate, and low (H, I, and L). Each group was further divided 
      into two subgroups: BDNF (1 mug) or control (artificial cerebrospinal fluid, 
      aCSF); they were then injected every other day for 20 days according to subgroup. 
      Energy intake, body weight, and body composition were measured. Other metabolic 
      indexes were measured before and after treatment. In parallel, another 12 rats 
      were fed control diet (CD), VMN-cannulated, and injected with aCSF. HFD exposure 
      induced obesity in the H group, with a significant increase in energy intake, 
      body weight, fat mass, liver size, and serum glucose, insulin, and leptin. BDNF 
      significantly reduced body weight and fat mass in all phenotypes, while it 
      reduced energy intake only in the I group. However, BDNF increased EE, 
      spontaneous physical activity, and fat oxidation in the H group, suggesting that 
      BDNF-induced EE elevation contributed to reduction of body weight and fat mass. 
      Chronic VMN BDNF reduced insulin elevation and/or reversed hyperleptinemia. These 
      data suggest that the VMN is an important site of action for BDNF reduction of 
      HFD-induced obesity.
CI  - Published by Elsevier Ltd.
FAU - Godar, R
AU  - Godar R
AD  - Veterans Affairs Medical Center, One Veterans Drive, Research Route 151, 
      Minneapolis, MN 55417, USA.
FAU - Dai, Y
AU  - Dai Y
FAU - Bainter, H
AU  - Bainter H
FAU - Billington, C
AU  - Billington C
FAU - Kotz, C M
AU  - Kotz CM
FAU - Wang, C F
AU  - Wang CF
LA  - eng
GR  - R01 DK080782/DK/NIDDK NIH HHS/United States
GR  - R01 DK080782-03/DK/NIDDK NIH HHS/United States
GR  - 1R01DK080782/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20110805
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Leptin)
SB  - IM
MH  - Adipose Tissue/physiology
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*physiology/therapeutic use
MH  - Contraindications
MH  - Diet, High-Fat/*adverse effects
MH  - Disease Models, Animal
MH  - Energy Intake/physiology
MH  - Hyperinsulinism/prevention & control
MH  - Leptin/antagonists & inhibitors/blood
MH  - Male
MH  - Obesity/*metabolism/physiopathology/*therapy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Ventromedial Hypothalamic Nucleus/*metabolism/physiology
PMC - PMC3190117
MID - NIHMS317270
EDAT- 2011/08/23 06:00
MHDA- 2012/06/26 06:00
PMCR- 2012/10/27
CRDT- 2011/08/23 06:00
PHST- 2011/01/28 00:00 [received]
PHST- 2011/06/24 00:00 [revised]
PHST- 2011/07/13 00:00 [accepted]
PHST- 2011/08/23 06:00 [entrez]
PHST- 2011/08/23 06:00 [pubmed]
PHST- 2012/06/26 06:00 [medline]
PHST- 2012/10/27 00:00 [pmc-release]
AID - S0306-4522(11)00925-0 [pii]
AID - 10.1016/j.neuroscience.2011.07.079 [doi]
PST - ppublish
SO  - Neuroscience. 2011 Oct 27;194:36-52. doi: 10.1016/j.neuroscience.2011.07.079. 
      Epub 2011 Aug 5.