PMID- 21857974
OWN - NLM
STAT- MEDLINE
DCOM- 20120215
LR  - 20211020
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 6
IP  - 8
DP  - 2011
TI  - Brain-derived neurotrophic factor in patients with Huntington's disease.
PG  - e22966
LID - 10.1371/journal.pone.0022966 [doi]
LID - e22966
AB  - Reduced Brain-Derived Neurotrophic Factor (BDNF) levels have been described in a 
      number of patho-physiological conditions, most notably, in Huntington's disease 
      (HD), a progressive neurodegenerative disorder. Since BDNF is also produced in 
      blood, we have undertaken the measurement of its peripheral levels in the attempt 
      to identify a possible link with HD prognosis and/or its progression. Here we 
      evaluated BDNF level in 398 blood samples including 138 controls, 56 preHD, and 
      204 HD subjects. We found that BDNF protein levels were not reliably different 
      between groups, whether measured in plasma (52 controls, 26 preHD, 105 HD) or 
      serum (39 controls, 5 preHD, 29 HD). Our experience, and a re-analysis of the 
      literature highlighted that intra-group variability and methodological aspects 
      affect this measurement, especially in serum. We also assessed BDNF mRNA levels 
      in blood samples from 47 controls, 25 preHD, and 70 HD subjects, and found no 
      differences among the groups. We concluded that levels of BDNF in human blood 
      were not informative (mRNA levels or plasma protein level) nor reliable (serum 
      protein levels) as HD biomarkers. We also wish to warn the scientific community 
      in interpreting the significance of changes measured in BDNF protein levels in 
      serum from patients suffering from different conditions.
FAU - Zuccato, Chiara
AU  - Zuccato C
AD  - Department of Pharmacological Sciences and Center for Stem Cell Research, 
      Universita degli Studi di Milano, Milan, Italy.
FAU - Marullo, Manuela
AU  - Marullo M
FAU - Vitali, Barbara
AU  - Vitali B
FAU - Tarditi, Alessia
AU  - Tarditi A
FAU - Mariotti, Caterina
AU  - Mariotti C
FAU - Valenza, Marta
AU  - Valenza M
FAU - Lahiri, Nayana
AU  - Lahiri N
FAU - Wild, Edward J
AU  - Wild EJ
FAU - Sassone, Jenny
AU  - Sassone J
FAU - Ciammola, Andrea
AU  - Ciammola A
FAU - Bachoud-Levi, Anne Catherine
AU  - Bachoud-Levi AC
FAU - Tabrizi, Sarah J
AU  - Tabrizi SJ
FAU - Di Donato, Stefano
AU  - Di Donato S
FAU - Cattaneo, Elena
AU  - Cattaneo E
LA  - eng
GR  - Department of Health/United Kingdom
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, Non-U.S. Gov't
DEP - 20110812
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (ANXA10 protein, human)
RN  - 0 (Annexins)
RN  - 0 (Antidepressive Agents, Tricyclic)
RN  - 0 (Antipsychotic Agents)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neurotransmitter Uptake Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 12794-10-4 (Benzodiazepines)
RN  - EC 2.7.11.1 (ROCK1 protein, human)
RN  - EC 2.7.11.1 (rho-Associated Kinases)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Annexins/genetics
MH  - Antidepressive Agents, Tricyclic/therapeutic use
MH  - Antipsychotic Agents/therapeutic use
MH  - Benzodiazepines/therapeutic use
MH  - Brain-Derived Neurotrophic Factor/*blood/*genetics
MH  - Cohort Studies
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - France
MH  - Gene Expression
MH  - Humans
MH  - Huntington Disease/*blood/drug therapy/*genetics
MH  - Italy
MH  - London
MH  - Male
MH  - Middle Aged
MH  - Neurotransmitter Uptake Inhibitors/therapeutic use
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Young Adult
MH  - rho-Associated Kinases/genetics
PMC - PMC3155522
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2011/08/23 06:00
MHDA- 2012/02/16 06:00
PMCR- 2011/08/12
CRDT- 2011/08/23 06:00
PHST- 2011/05/24 00:00 [received]
PHST- 2011/07/03 00:00 [accepted]
PHST- 2011/08/23 06:00 [entrez]
PHST- 2011/08/23 06:00 [pubmed]
PHST- 2012/02/16 06:00 [medline]
PHST- 2011/08/12 00:00 [pmc-release]
AID - PONE-D-11-09103 [pii]
AID - 10.1371/journal.pone.0022966 [doi]
PST - ppublish
SO  - PLoS One. 2011;6(8):e22966. doi: 10.1371/journal.pone.0022966. Epub 2011 Aug 12.