PMID- 21858036
OWN - NLM
STAT- MEDLINE
DCOM- 20120215
LR  - 20211020
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 6
IP  - 8
DP  - 2011
TI  - The influence of Staphylococcus aureus on gut microbial ecology in an in vitro 
      continuous culture human colonic model system.
PG  - e23227
LID - 10.1371/journal.pone.0023227 [doi]
LID - e23227
AB  - An anaerobic three-stage continuous culture model of the human colon (gut model), 
      which represent different anatomical areas of the large intestine, was used to 
      study the effect of S. aureus infection of the gut on the resident faecal 
      microbiota. Studies on the development of the microbiota in the three vessels 
      were performed and bacteria identified by culture independent fluorescence in 
      situ hybridization (FISH). Furthermore, short chain fatty acids (SCFA), as 
      principal end products of gut bacterial metabolism, were measured along with a 
      quantitative assessment of the predominant microbiota. During steady state 
      conditions, numbers of S. aureus cells stabilised until they were washed out, but 
      populations of indigenous bacteria were transiently altered; thus S. aureus was 
      able to compromise colonisation resistance by the colonic microbiota. 
      Furthermore, the concentration of butyric acid in the vessel representing the 
      proximal colon was significantly decreased by infection. Thus infection by S. 
      aureus appears to be able to alter the overall structure of the human colonic 
      microbiota and the microbial metabolic profiles. This work provides an initial in 
      vitro model to analyse interactions with pathogens.
FAU - Sannasiddappa, Thippeswamy H
AU  - Sannasiddappa TH
AD  - School of Biological Sciences, University of Reading, Reading, United Kingdom.
FAU - Costabile, Adele
AU  - Costabile A
FAU - Gibson, Glenn R
AU  - Gibson GR
FAU - Clarke, Simon R
AU  - Clarke SR
LA  - eng
PT  - Journal Article
DEP - 20110809
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Fatty Acids, Volatile)
RN  - 107-92-6 (Butyric Acid)
SB  - IM
MH  - Bacteria/genetics/*growth & development/metabolism
MH  - Butyric Acid/metabolism
MH  - Colon/*microbiology
MH  - *Ecosystem
MH  - Fatty Acids, Volatile/metabolism
MH  - Gastrointestinal Tract/*microbiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Metagenome/genetics
MH  - Models, Biological
MH  - Staphylococcal Infections/microbiology
MH  - Staphylococcus aureus/genetics/*growth & development/metabolism
MH  - Time Factors
MH  - Tissue Culture Techniques/instrumentation/methods
PMC - PMC3153491
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2011/08/23 06:00
MHDA- 2012/02/16 06:00
PMCR- 2011/08/09
CRDT- 2011/08/23 06:00
PHST- 2011/03/16 00:00 [received]
PHST- 2011/07/13 00:00 [accepted]
PHST- 2011/08/23 06:00 [entrez]
PHST- 2011/08/23 06:00 [pubmed]
PHST- 2012/02/16 06:00 [medline]
PHST- 2011/08/09 00:00 [pmc-release]
AID - PONE-D-11-05042 [pii]
AID - 10.1371/journal.pone.0023227 [doi]
PST - ppublish
SO  - PLoS One. 2011;6(8):e23227. doi: 10.1371/journal.pone.0023227. Epub 2011 Aug 9.