PMID- 21861285
OWN - NLM
STAT- MEDLINE
DCOM- 20120130
LR  - 20220311
IS  - 1555-4317 (Electronic)
IS  - 1555-4309 (Print)
IS  - 1555-4309 (Linking)
VI  - 6
IP  - 4
DP  - 2011 Jul-Aug
TI  - Use of perfluorocarbon nanoparticles for non-invasive multimodal cell tracking of 
      human pancreatic islets.
PG  - 251-9
LID - 10.1002/cmmi.424 [doi]
AB  - In vivo imaging of engraftment and immunorejection of transplanted islets is 
      critical for further clinical development, with (1)H MR imaging of 
      superparamagnetic iron oxide-labeled cells being the current premier modality. 
      Using perfluorocarbon nanoparticles, we present here a strategy for non-invasive 
      imaging of cells using other modalities. To this end, human cadaveric islets were 
      labeled with rhodamine-perfluorooctylbromide (PFOB) nanoparticles, 
      rhodamine-perfluoropolyether (PFPE) nanoparticles or Feridex as control and 
      tested in vitro for cell viability and c-peptide secretion for 1 week. (19)F MRI, 
      computed tomography (CT) and ultrasound (US) imaging was performed on labeled 
      cell phantoms and on cells following transplantation beneath the kidney capsule 
      of mice and rabbits. PFOB and PFPE-labeling did not reduce human islet viability 
      or glucose responsiveness as compared with unlabeled cells or SPIO-labeled cells. 
      PFOB- and PFPE-labeled islets were effectively fluorinated for visualization by 
      (19)F MRI. PFOB-labeled islets were acoustically reflective for detection by US 
      imaging and became sufficiently brominated to become radiopaque allowing 
      visualization with CT. Thus, perfluorocarbon nanoparticles are multimodal 
      cellular contrast agents that may find applications in real-time targeted 
      delivery and imaging of transplanted human islets or other cells in a clinically 
      applicable manner using MRI, US or CT imaging.
CI  - Copyright (c) 2011 John Wiley & Sons, Ltd.
FAU - Barnett, Brad P
AU  - Barnett BP
AD  - Department of Radiology and Radiological Science, Division of MR Research, The 
      Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
FAU - Ruiz-Cabello, Jesus
AU  - Ruiz-Cabello J
FAU - Hota, Partha
AU  - Hota P
FAU - Ouwerkerk, Ronald
AU  - Ouwerkerk R
FAU - Shamblott, Michael J
AU  - Shamblott MJ
FAU - Lauzon, Cal
AU  - Lauzon C
FAU - Walczak, Piotr
AU  - Walczak P
FAU - Gilson, Wesley D
AU  - Gilson WD
FAU - Chacko, Vadappuram P
AU  - Chacko VP
FAU - Kraitchman, Dara L
AU  - Kraitchman DL
FAU - Arepally, Aravind
AU  - Arepally A
FAU - Bulte, Jeff W M
AU  - Bulte JW
LA  - eng
GR  - R01 EB007825/EB/NIBIB NIH HHS/United States
GR  - R01 EB007825-02/EB/NIBIB NIH HHS/United States
GR  - R01 EB007825-03/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PL  - England
TA  - Contrast Media Mol Imaging
JT  - Contrast media & molecular imaging
JID - 101286760
RN  - 0 (Fluorocarbons)
SB  - IM
MH  - Animals
MH  - Cell Tracking/*methods
MH  - Fluorocarbons/*chemistry
MH  - Humans
MH  - In Vitro Techniques
MH  - Islets of Langerhans/*cytology
MH  - Magnetic Resonance Imaging/methods
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nanoparticles/*chemistry
MH  - Rabbits
PMC - PMC3160722
MID - NIHMS261831
EDAT- 2011/08/24 06:00
MHDA- 2012/01/31 06:00
PMCR- 2012/07/01
CRDT- 2011/08/24 06:00
PHST- 2011/08/24 06:00 [entrez]
PHST- 2011/08/24 06:00 [pubmed]
PHST- 2012/01/31 06:00 [medline]
PHST- 2012/07/01 00:00 [pmc-release]
AID - 10.1002/cmmi.424 [doi]
PST - ppublish
SO  - Contrast Media Mol Imaging. 2011 Jul-Aug;6(4):251-9. doi: 10.1002/cmmi.424.