PMID- 21862514
OWN - NLM
STAT- MEDLINE
DCOM- 20130326
LR  - 20220317
IS  - 1530-8022 (Electronic)
IS  - 0885-3282 (Linking)
VI  - 27
IP  - 4
DP  - 2012 Nov
TI  - Effects of dexamethasone-loaded PLGA microspheres on human fetal osteoblasts.
PG  - 477-83
LID - 10.1177/0885328211412634 [doi]
AB  - Integration of a drug delivery function into implantable medical devices enables 
      local release of specific bioactives to control cells-surface interactions. One 
      alternative to achieve this biofunctionality for bone implants is to incorporate 
      particulate drug delivery systems (DDSs) into the rough or porous implant 
      surfaces. The scope of this study was to assess the effects of a model DDS 
      consisting of poly(D,L-lactide-co-glycolide) (PLGA) microspheres loaded with an 
      anti-inflammatory drug, dexamethasone (DXM), on the response of Simian 
      Virus-immortalized Human Fetal Osteoblast (SV-HFO) cells. The microspheres were 
      prepared by the oil-in-water emulsion/solvent evaporation method, whereas cells 
      response was investigated by Alamar Blue test for viability, alkaline phosphatase 
      (ALP) activity for differentiation, and Alizarin Red staining for matrix 
      mineralization. Cell viability was not affected by the presence of increased 
      concentrations of polymeric microspheres in the culture media. Furthermore, in 
      the cultures with DXM-loaded microspheres, ALP activity was expressed at levels 
      similar with those obtained under osteogenic conditions, indicating that DXM 
      released from the microsphere-stimulated cell differentiation. Matrix 
      mineralization occurred preferentially around the DXM-loaded microspheres 
      confirming that the released DXM could act as osteogenic supplement for the 
      cells. These in vitro findings suggest that a particulate PLGA-DXM DDS may 
      actually provide dual, anti-inflammatory and osteogenic functions when 
      incorporated on the surface of bone implants.
FAU - Dawes, G J S
AU  - Dawes GJ
AD  - Department of Biomechanical Engineering, Faculty of Mechanical, Maritime and 
      Materials Engineering, Delft University of Technology, Mekelweg 2, 2628 CD Delft, 
      The Netherlands.
FAU - Fratila-Apachitei, L E
AU  - Fratila-Apachitei LE
FAU - Necula, B S
AU  - Necula BS
FAU - Apachitei, I
AU  - Apachitei I
FAU - van Leeuwen, J P T M
AU  - van Leeuwen JP
FAU - Duszczyk, J
AU  - Duszczyk J
FAU - Eijken, M
AU  - Eijken M
LA  - eng
PT  - Journal Article
DEP - 20110823
PL  - England
TA  - J Biomater Appl
JT  - Journal of biomaterials applications
JID - 8813912
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Coated Materials, Biocompatible)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Anti-Inflammatory Agents/*administration & dosage/pharmacology
MH  - Cell Differentiation/drug effects
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Coated Materials, Biocompatible/*chemistry/metabolism
MH  - Dexamethasone/*administration & dosage/pharmacology
MH  - *Drug Delivery Systems
MH  - Humans
MH  - Lactic Acid/*chemistry/metabolism
MH  - Microspheres
MH  - Osteoblasts/cytology/*drug effects/metabolism
MH  - Polyglycolic Acid/*chemistry/metabolism
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Prostheses and Implants
EDAT- 2011/08/25 06:00
MHDA- 2013/03/27 06:00
CRDT- 2011/08/25 06:00
PHST- 2011/08/25 06:00 [entrez]
PHST- 2011/08/25 06:00 [pubmed]
PHST- 2013/03/27 06:00 [medline]
AID - 0885328211412634 [pii]
AID - 10.1177/0885328211412634 [doi]
PST - ppublish
SO  - J Biomater Appl. 2012 Nov;27(4):477-83. doi: 10.1177/0885328211412634. Epub 2011 
      Aug 23.