PMID- 21863333
OWN - NLM
STAT- MEDLINE
DCOM- 20120917
LR  - 20211020
IS  - 1573-5168 (Electronic)
IS  - 0920-1742 (Linking)
VI  - 38
IP  - 3
DP  - 2012 Jun
TI  - Effects of low environmental salinity on the cellular profiles and expression of 
      Na+, K+-ATPase and Na+, K+, 2Cl- cotransporter 1 of branchial mitochondrion-rich 
      cells in the juvenile marine fish Monodactylus argenteus.
PG  - 665-78
LID - 10.1007/s10695-011-9549-1 [doi]
AB  - The goal of this study was to determine the osmoregulatory ability of a juvenile 
      marine fish, silver moony (Monodactylus argenteus), for the purpose of developing 
      a new experimental species for ecophysiological research. In this study, M. 
      argenteus was acclimated to freshwater (FW), brackish water (BW), or seawater 
      (SW). The salinity tolerance of this euryhaline species was effective, and the 
      fish survived well upon osmotic challenges. The largest apical surface of 
      mitochondrion-rich cells was found in the FW individuals. Immunohistochemical 
      staining revealed that Na(+), K(+)-ATPase immunoreactive (NKA-IR) cells were 
      distributed in the interlamellar region of the gill filaments of the silver moony 
      in all experimental groups. In addition to the filaments, NKA-IR cells were also 
      found in the lamellae of the FW individuals. The number of NKA-IR cells in the 
      gills of the FW individuals exceeded that of the BW and SW individuals. The 
      NKA-IR cells of FW and SW individuals exhibited bigger size than that of BW fish. 
      The NKA activities and protein expression of the NKA alpha-subunit in the gills of 
      the FW individuals were significantly higher than in the BW and SW groups. 
      Additionally, the relative amounts of Na(+), K(+), 2Cl(-) cotransporter 1 (NKCC1) 
      were salinity-dependent in the gills. Immunofluorescent signals of NKCC1 were 
      localized to the basolateral membrane of NKA-IR cells in all groups. In the gills 
      of the FW individuals, however, some NKA-IR cells did not exhibit a basolateral 
      NKCC1 signal. In conclusion, the present study illustrated the osmoregulatory 
      mechanisms of this easy- and economic-to-rear marine teleost with euryhaline 
      capacity and proved the silver moony to be a good experimental animal.
FAU - Kang, Chao-Kai
AU  - Kang CK
AD  - Department of Life Sciences, National Chung Hsing University, Taichung 402, 
      Taiwan.
FAU - Liu, Fu-Chen
AU  - Liu FC
FAU - Chang, Wen-Been
AU  - Chang WB
FAU - Lee, Tsung-Han
AU  - Lee TH
LA  - eng
GR  - N01-HD-62915/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110824
PL  - Netherlands
TA  - Fish Physiol Biochem
JT  - Fish physiology and biochemistry
JID - 100955049
RN  - 0 (Sodium-Potassium-Chloride Symporters)
RN  - 0 (Solute Carrier Family 12, Member 2)
RN  - EC 7.2.2.13 (Sodium-Potassium-Exchanging ATPase)
SB  - IM
MH  - Acclimatization/physiology
MH  - Animals
MH  - Branchial Region/metabolism/ultrastructure
MH  - Fresh Water/analysis
MH  - Gills/metabolism/ultrastructure
MH  - Immunohistochemistry
MH  - Microscopy, Electron, Scanning
MH  - Mitochondria/metabolism
MH  - Perciformes/anatomy & histology/*metabolism
MH  - Salinity
MH  - Seawater/analysis
MH  - Sodium-Potassium-Chloride Symporters/*metabolism
MH  - Sodium-Potassium-Exchanging ATPase/*metabolism
MH  - Solute Carrier Family 12, Member 2
MH  - Water-Electrolyte Balance/physiology
EDAT- 2011/08/25 06:00
MHDA- 2012/09/18 06:00
CRDT- 2011/08/25 06:00
PHST- 2011/02/09 00:00 [received]
PHST- 2011/08/06 00:00 [accepted]
PHST- 2011/08/25 06:00 [entrez]
PHST- 2011/08/25 06:00 [pubmed]
PHST- 2012/09/18 06:00 [medline]
AID - 10.1007/s10695-011-9549-1 [doi]
PST - ppublish
SO  - Fish Physiol Biochem. 2012 Jun;38(3):665-78. doi: 10.1007/s10695-011-9549-1. Epub 
      2011 Aug 24.