PMID- 21864136
OWN - NLM
STAT- MEDLINE
DCOM- 20120501
LR  - 20211020
IS  - 1473-0804 (Electronic)
IS  - 1369-7137 (Print)
IS  - 1369-7137 (Linking)
VI  - 15
IP  - 1
DP  - 2012 Feb
TI  - Effect of ovarian aging on androgen biosynthesis in a cynomolgus macaque model.
PG  - 82-92
LID - 10.3109/13697137.2011.571321 [doi]
AB  - OBJECTIVE: The role of androgens in chronic disease pathogenesis, cognitive 
      function and libido during menopause is of increasing interest. The aim of this 
      study was to characterize the distribution and expression of androgenic proteins 
      in the macaque ovary and to investigate the relationship between serum androgen 
      concentrations, follicle number, and the persistence of androgenesis in the aging 
      macaque ovary. METHODS: The subjects were 26 adult female cynomolgus macaques. 
      Ovaries were immunostained for cytochrome P450 17alpha-hydroxylase/17-20 lyase 
      (P450c17), 3beta-hydroxysteroid dehydrogenase (3betaHSD), and cytochrome b5 (cytb5). 
      Based on primordial follicle counts, animals were divided into tertiles (low 
      (</=200), intermediate (226-1232), and high (2372-4356)) to evaluate differences in 
      androgen staining and changes in serum androgen concentrations following 
      ovariectomy. RESULTS: Positive immunostaining for P450c17 and cytb5 within the 
      theca interna layer of growing follicles persisted in advanced atretic follicles 
      and secondary interstitial cells (residual stromal cells). Ovaries with low 
      follicle numbers had less staining for all androgenic proteins compared to 
      ovaries with higher numbers of growing follicles. Immunostaining for cytb5 was 
      the most reliable marker for persistent androgenesis in ovaries with minimal 
      primordial follicle numbers (<100) and residual stromal cells. Following 
      ovariectomy, a significant decrease in testosterone (-27.7%, -30.8%, -27.5%; p < 
      0.01) and androstenedione (-33.4%, -35.7%, -46.0%; p < 0.01) was observed in 
      monkeys with low, intermediate, and high primordial follicle counts, 
      respectively. CONCLUSIONS: Despite low follicle numbers, the aging macaque ovary 
      retains the necessary proteins for androgenesis within residual stromal cells and 
      contributes to peripheral androgen concentrations.
FAU - Ethun, K F
AU  - Ethun KF
AD  - Department of Pathology/Comparative Medicine, Wake Forest University School of 
      Medicine, Winston-Salem, NC 27157-1040, USA.
FAU - Wood, C E
AU  - Wood CE
FAU - Parker, C R Jr
AU  - Parker CR Jr
FAU - Kaplan, J R
AU  - Kaplan JR
FAU - Chen, H
AU  - Chen H
FAU - Appt, S E
AU  - Appt SE
LA  - eng
GR  - R03-AG20290/AG/NIA NIH HHS/United States
GR  - R01 AG 027847/AG/NIA NIH HHS/United States
GR  - R01 AG027847/AG/NIA NIH HHS/United States
GR  - K01 RR 021322-05/RR/NCRR NIH HHS/United States
GR  - T32 RR07009-32/RR/NCRR NIH HHS/United States
GR  - K01 RR021322/RR/NCRR NIH HHS/United States
GR  - T32 OD010957/OD/NIH HHS/United States
GR  - R01 HL079421/HL/NHLBI NIH HHS/United States
GR  - T32 RR007009/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20110824
PL  - England
TA  - Climacteric
JT  - Climacteric : the journal of the International Menopause Society
JID - 9810959
RN  - 0 (Androgens)
RN  - 0 (Coloring Agents)
RN  - 9035-39-6 (Cytochromes b5)
RN  - EC 1.1.- (3-Hydroxysteroid Dehydrogenases)
RN  - EC 1.14.14.19 (Steroid 17-alpha-Hydroxylase)
SB  - IM
MH  - 3-Hydroxysteroid Dehydrogenases/*metabolism
MH  - Androgens/*biosynthesis/blood
MH  - Animals
MH  - Cellular Senescence
MH  - Coloring Agents/metabolism
MH  - Cytochromes b5/*metabolism
MH  - Female
MH  - Immunohistochemistry/methods
MH  - Macaca fascicularis
MH  - Models, Animal
MH  - Monitoring, Physiologic
MH  - Ovariectomy/adverse effects
MH  - Postoperative Care/methods
MH  - Preoperative Care/methods
MH  - Steroid 17-alpha-Hydroxylase/*metabolism
MH  - Stromal Cells/metabolism
MH  - *Theca Cells/cytology/metabolism
PMC - PMC3668306
MID - NIHMS472583
COIS- Conflict of interest The authors report no conflict of interest. The authors 
      alone are responsible for the content and writing of the paper.
EDAT- 2011/08/26 06:00
MHDA- 2012/05/02 06:00
PMCR- 2013/05/31
CRDT- 2011/08/26 06:00
PHST- 2011/08/26 06:00 [entrez]
PHST- 2011/08/26 06:00 [pubmed]
PHST- 2012/05/02 06:00 [medline]
PHST- 2013/05/31 00:00 [pmc-release]
AID - 10.3109/13697137.2011.571321 [doi]
PST - ppublish
SO  - Climacteric. 2012 Feb;15(1):82-92. doi: 10.3109/13697137.2011.571321. Epub 2011 
      Aug 24.