PMID- 21870113
OWN - NLM
STAT- MEDLINE
DCOM- 20120130
LR  - 20240204
IS  - 0219-1032 (Electronic)
IS  - 1016-8478 (Print)
IS  - 1016-8478 (Linking)
VI  - 32
IP  - 3
DP  - 2011 Sep
TI  - Pim protein kinase-3 is regulated by TNF-alpha and promotes endothelial cell 
      sprouting.
PG  - 235-41
LID - 10.1007/s10059-011-1026-z [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) plays an important role in pathological 
      angiogenesis associated with inflammatory response. Pim-3 kinase belonging to 
      serine/threonine protein kinases is a potent suppressor of myc-induced apoptosis. 
      We have recently demonstrated that Pim-3 plays an essential role in endothelial 
      cell (EC) spreading and migration. In this study, we showed that TNF-alpha 
      transiently increased Pim-3 mRNA expression, and this was mediated through Tumor 
      necrosis factor-alpha receptor-1 (TNFR1) pathway in ECs. TNF-alpha could promote 
      stabilization of Pim- 3 mRNA in ECs. Small-interfering RNA (siRNA)-mediated gene 
      knockdown of Pim-3 significantly impaired TNF-alpha-induced formation of EC membrane 
      protrusions in vitro. Furthermore, Pim-3 silencing inhibited EC sprouting in 
      subcutaneous Matrigel in vivo. eNOS mRNA abundance was lower in Pim-3 siRNA 
      transfected ECs compared with the control ECs. These observations suggest that 
      Pim-3 plays a role in TNF-alpha-induced angiogenesis.
FAU - Yang, Handong
AU  - Yang H
AD  - Department of Cardiovascular Diseases, Renmin Hospital of Wuhan University, Wuhan 
      City, China.
FAU - Wang, Yinfang
AU  - Wang Y
FAU - Qian, Hang
AU  - Qian H
FAU - Zhang, Peng
AU  - Zhang P
FAU - Huang, Congxin
AU  - Huang C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110823
PL  - United States
TA  - Mol Cells
JT  - Molecules and cells
JID - 9610936
RN  - 0 (Drug Combinations)
RN  - 0 (Laminin)
RN  - 0 (Proteoglycans)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type I)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 119978-18-6 (matrigel)
RN  - 9007-34-5 (Collagen)
RN  - EC 1.14.13.39 (NOS3 protein, human)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
RN  - EC 2.7.11.1 (PIM3 protein, human)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Blotting, Western
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Collagen/chemistry/metabolism
MH  - Drug Combinations
MH  - Female
MH  - Gene Expression
MH  - Gene Silencing/drug effects
MH  - Human Umbilical Vein Endothelial Cells/cytology/*metabolism
MH  - Humans
MH  - Laminin/chemistry/metabolism
MH  - Neoplasms/*blood supply/pathology
MH  - Neovascularization, Pathologic/genetics/*metabolism
MH  - Nitric Oxide Synthase Type III/genetics/*metabolism
MH  - Protein Serine-Threonine Kinases/antagonists & inhibitors/genetics/*metabolism
MH  - Proteoglycans/chemistry/metabolism
MH  - Proto-Oncogene Proteins/antagonists & inhibitors/genetics/*metabolism
MH  - RNA Stability
MH  - RNA, Messenger/analysis/biosynthesis
MH  - RNA, Small Interfering/pharmacology
MH  - Receptors, Tumor Necrosis Factor, Type I/genetics/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/genetics/*metabolism
MH  - Up-Regulation
PMC - PMC3887632
EDAT- 2011/08/27 06:00
MHDA- 2012/01/31 06:00
PMCR- 2012/09/30
CRDT- 2011/08/27 06:00
PHST- 2011/02/14 00:00 [received]
PHST- 2011/06/29 00:00 [accepted]
PHST- 2011/06/28 00:00 [revised]
PHST- 2011/08/27 06:00 [entrez]
PHST- 2011/08/27 06:00 [pubmed]
PHST- 2012/01/31 06:00 [medline]
PHST- 2012/09/30 00:00 [pmc-release]
AID - S1016-8478(23)10950-2 [pii]
AID - ksmcb-32-235 [pii]
AID - 10.1007/s10059-011-1026-z [doi]
PST - ppublish
SO  - Mol Cells. 2011 Sep;32(3):235-41. doi: 10.1007/s10059-011-1026-z. Epub 2011 Aug 
      23.