PMID- 21870155
OWN - NLM
STAT- MEDLINE
DCOM- 20130124
LR  - 20211020
IS  - 1559-1166 (Electronic)
IS  - 0895-8696 (Linking)
VI  - 46
IP  - 1
DP  - 2012 Jan
TI  - Activity and expression of enkephalinase and aminopeptidase N in regions of the 
      mesocorticolimbic system are selectively modified by acute ethanol 
      administration.
PG  - 58-67
LID - 10.1007/s12031-011-9623-2 [doi]
AB  - Opioid peptides play a key role in ethanol reinforcement and alcohol drinking 
      behavior. However, regulation of opioid levels by peptidase-degrading activities 
      in ethanol's actions in brain is still unclear. The aim of this work was to study 
      the acute effects of ethanol (2.5 g/kg) on enkephalinase (NEP) and aminopeptidase 
      N (APN) activities and expression in regions of the mesocorticolimbic system, as 
      well as on corticosterone levels in serum for up to 24 h after administration. 
      Enzymatic activities were measured by fluorometric assays, mRNA's expression by 
      reverse transcriptase polymerase chain reaction (RT-PCR) and corticosterone 
      levels by radioimmunoassay. Acute ethanol administration modified peptidase 
      activity and expression with different kinetics. Ethanol induced a transitory 
      increase and decrease in NEP and APN activities in the frontal cortex (FC) and 
      ventral tegmental area (VTA), whereas only increases in these activities were 
      observed in the nucleus accumbens (NAcc). Ethanol induced an increase in NEP mRNA 
      in the FC and decreases in APN mRNA in the FC and NAcc. In contrast, ethanol 
      produced biphasic effects on both enzymes expression in the VTA. Corticosterone 
      levels were not changed by ethanol. Our results suggest that NEP and APN could 
      play a main role in ethanol reinforcement through regulation of opioid levels in 
      mesolimbic areas.
FAU - Morales-Mulia, Marcela
AU  - Morales-Mulia M
AD  - Departamento de Neuroquimica, Subdireccion de Investigaciones Clinicas, Instituto 
      Nacional de Psiquiatria Ramon de la Fuente, Calzada Mexico-Xochimilco 101, Col. 
      San Lorenzo Huipulco, 14370, Mexico, DF, Mexico.
FAU - de Gortari, Patricia
AU  - de Gortari P
FAU - Amaya, Maria-Isabel
AU  - Amaya MI
FAU - Mendez, Milagros
AU  - Mendez M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110826
PL  - United States
TA  - J Mol Neurosci
JT  - Journal of molecular neuroscience : MN
JID - 9002991
RN  - 3K9958V90M (Ethanol)
RN  - EC 3.4.11.2 (CD13 Antigens)
RN  - EC 3.4.24.11 (Neprilysin)
SB  - IM
MH  - Acute Disease
MH  - Alcohol Drinking/*metabolism
MH  - Alcohol-Induced Disorders, Nervous System/*metabolism
MH  - Animals
MH  - CD13 Antigens/biosynthesis/*genetics
MH  - Ethanol/*toxicity
MH  - Limbic System/drug effects/*enzymology
MH  - Male
MH  - Neprilysin/biosynthesis/*genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Ventral Tegmental Area/drug effects/*enzymology
EDAT- 2011/08/27 06:00
MHDA- 2013/01/25 06:00
CRDT- 2011/08/27 06:00
PHST- 2011/06/09 00:00 [received]
PHST- 2011/08/08 00:00 [accepted]
PHST- 2011/08/27 06:00 [entrez]
PHST- 2011/08/27 06:00 [pubmed]
PHST- 2013/01/25 06:00 [medline]
AID - 10.1007/s12031-011-9623-2 [doi]
PST - ppublish
SO  - J Mol Neurosci. 2012 Jan;46(1):58-67. doi: 10.1007/s12031-011-9623-2. Epub 2011 
      Aug 26.