PMID- 21871621
OWN - NLM
STAT- MEDLINE
DCOM- 20111129
LR  - 20220317
IS  - 1556-5653 (Electronic)
IS  - 0015-0282 (Linking)
VI  - 96
IP  - 4
DP  - 2011 Oct
TI  - Sperm fluorescence in situ hybridization study in nine men carrying a 
      Robertsonian or a reciprocal translocation: relationship between segregation 
      modes and high-magnification sperm morphology examination.
PG  - 826-32
LID - 10.1016/j.fertnstert.2011.07.1143 [doi]
AB  - OBJECTIVE: To evaluate whether observation of spermatozoa at x 6,100 
      magnification can distinguish between those with and without a balanced 
      chromosomal content. DESIGN: Retrospective research study. SETTING: Genetics 
      laboratory of a university hospital and in vitro fertilization center. 
      PATIENT(S): Six men carrying a reciprocal translocation and three men with a 
      Robertsonian translocation. INTERVENTION(S): Sperm fluorescence in situ 
      hybridization (FISH) with a specific set of three probes for each translocation 
      for determining chromosomal content, performed on both unselected spermatozoa and 
      on spermatozoa selected at x 6,100 magnification according to the Cassuto-Barak 
      classification. MAIN OUTCOME MEASURE(S): Chromosomal content in unselected and 
      selected spermatozoa. RESULT(S): Chromosomal translocations lead to gametes 
      carrying either a balanced or an unbalanced karyotype in offspring and 
      consequently to changes in chromosome position within sperm nucleus and 
      potentially in nuclear morphology. In the unselected spermatozoa, the rate of 
      chromosomally balanced nuclei ranged from 37.1% to 52.6% and from 70% to 88.6% in 
      reciprocal and Robertsonian translocations, respectively, which is in agreement 
      with published data. In selected spermatozoa, there was no statistically 
      significant difference between the rates of segregation modes when compared with 
      their frequencies in unselected sperm cells. CONCLUSION(S): The observation of 
      spermatozoa at high-magnification in translocation carriers cannot be used to 
      select sperm cells with a balanced chromosomal content.
CI  - Copyright (c) 2011 American Society for Reproductive Medicine. Published by 
      Elsevier Inc. All rights reserved.
FAU - Cassuto, Nino Guy
AU  - Cassuto NG
AD  - ART Unit, Drouot Laboratory, Paris, France.
FAU - Le Foll, Nathalie
AU  - Le Foll N
FAU - Chantot-Bastaraud, Sandra
AU  - Chantot-Bastaraud S
FAU - Balet, Richard
AU  - Balet R
FAU - Bouret, Dominique
AU  - Bouret D
FAU - Rouen, Alexandre
AU  - Rouen A
FAU - Bhouri, Rakia
AU  - Bhouri R
FAU - Hyon, Capucine
AU  - Hyon C
FAU - Siffroi, Jean Pierre
AU  - Siffroi JP
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20110825
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
SB  - IM
MH  - Adult
MH  - Chromosome Segregation/*genetics
MH  - Genetic Carrier Screening/methods
MH  - *Heterozygote
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Infertility, Male/diagnosis/*genetics
MH  - Male
MH  - Retrospective Studies
MH  - Spermatozoa/chemistry/*physiology
MH  - Translocation, Genetic/*genetics
EDAT- 2011/08/30 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/08/30 06:00
PHST- 2011/04/29 00:00 [received]
PHST- 2011/07/11 00:00 [revised]
PHST- 2011/07/26 00:00 [accepted]
PHST- 2011/08/30 06:00 [entrez]
PHST- 2011/08/30 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - S0015-0282(11)02259-X [pii]
AID - 10.1016/j.fertnstert.2011.07.1143 [doi]
PST - ppublish
SO  - Fertil Steril. 2011 Oct;96(4):826-32. doi: 10.1016/j.fertnstert.2011.07.1143. 
      Epub 2011 Aug 25.