PMID- 21872626
OWN - NLM
STAT- MEDLINE
DCOM- 20120206
LR  - 20111017
IS  - 1879-1166 (Electronic)
IS  - 0198-8859 (Linking)
VI  - 72
IP  - 11
DP  - 2011 Nov
TI  - Mismatches outside exons 2 and 3 do not alter the peptide motif of the allele 
      group B*44:02P.
PG  - 1039-44
LID - 10.1016/j.humimm.2011.08.004 [doi]
AB  - Sequence variations outside exons 2 and 3 do not appear to affect the function of 
      human leukocyte antigen (HLA) class I alleles. HLA-B*44:02:01:01 and -B*44:27 are 
      considered functionally identical because they differ by a single amino acid 
      substitution of Val > Ala at position 199, which is located in the alpha3 domain. To 
      validate that HLA-B*44:02:01:01 and -B*44:27 represent functionally identical 
      alleles that might reflect a permissive mismatch in hematopoetic stem cell 
      transplantation (HSCT), we determined their peptide-binding features. 
      B-lymphoblastic cells were lentivirally transduced with B*44:02 and B*44:27 
      constructs and soluble recombinant molecules were purified by affinity 
      chromatography. Peptides were isolated and sequencing of single peptides was 
      performed using liquid chromatography-electrospray ionization-tandem mass 
      spectrometry (LTQ-Orbitrap) technology. We demonstrate that the peptide motif of 
      B*44:02(199Val) and B*44:27(199Ala) is identical. Both variants feature E at P2 
      and Y, F, or W at POmega in their ligands. Most of the identified peptides are 9 to 
      11 amino acids in length and approximately 20% of these ligands are shared 
      between the alleles. Our results lead to the conclusion that B*44:02:01:01 and 
      B*44:27 might have the same immune function, validating a theory that is now 
      being used in deciding which donors to select in HSCT when there is no identical 
      donor available.
CI  - Copyright (c) 2011 American Society for Histocompatibility and Immunogenetics. All 
      rights reserved.
FAU - Bade-Doeding, Christina
AU  - Bade-Doeding C
AD  - Institute for Transfusion Medicine, Hannover Medical School, 30625 Hannover, 
      Germany. bade-doeding.christina@mh-hannover.de
FAU - Cano, Pedro
AU  - Cano P
FAU - Huyton, Trevor
AU  - Huyton T
FAU - Badrinath, Soumya
AU  - Badrinath S
FAU - Eiz-Vesper, Britta
AU  - Eiz-Vesper B
FAU - Hiller, Oliver
AU  - Hiller O
FAU - Blasczyk, Rainer
AU  - Blasczyk R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110810
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-B*44:27 antigen)
RN  - 0 (HLA-B44 Antigen)
RN  - 0 (Peptide Fragments)
SB  - IM
MH  - Amino Acid Motifs/genetics
MH  - B-Lymphocytes/cytology/immunology/*metabolism
MH  - Cell Line, Tumor
MH  - Chromatography, Affinity
MH  - Donor Selection
MH  - Exons/genetics
MH  - HLA Antigens/chemistry/genetics/*metabolism
MH  - HLA-B Antigens/genetics/metabolism
MH  - HLA-B44 Antigen/chemistry/genetics/*metabolism
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Histocompatibility/immunology
MH  - Humans
MH  - Mutation/genetics
MH  - Peptide Fragments/genetics/immunology/*metabolism
MH  - Polymorphism, Genetic
MH  - Protein Binding/genetics/immunology
MH  - Tandem Mass Spectrometry
MH  - Transgenes/genetics
EDAT- 2011/08/30 06:00
MHDA- 2012/02/07 06:00
CRDT- 2011/08/30 06:00
PHST- 2011/01/20 00:00 [received]
PHST- 2011/07/31 00:00 [revised]
PHST- 2011/08/04 00:00 [accepted]
PHST- 2011/08/30 06:00 [entrez]
PHST- 2011/08/30 06:00 [pubmed]
PHST- 2012/02/07 06:00 [medline]
AID - S0198-8859(11)00493-9 [pii]
AID - 10.1016/j.humimm.2011.08.004 [doi]
PST - ppublish
SO  - Hum Immunol. 2011 Nov;72(11):1039-44. doi: 10.1016/j.humimm.2011.08.004. Epub 
      2011 Aug 10.