PMID- 21875260
OWN - NLM
STAT- MEDLINE
DCOM- 20120529
LR  - 20221207
IS  - 2476-762X (Electronic)
IS  - 1513-7368 (Linking)
VI  - 12
IP  - 5
DP  - 2011
TI  - Clinical significance of human telomerase RNA gene (hTERC) amplification in 
      cervical squamous cell lesions detected by fluorescence in situ hybridization.
PG  - 1167-71
AB  - BACKGROUND: Genomic amplification of the human telomerase RNA gene (hTERC), 
      located in the chromosome 3q26 region, has been documented in tumorigenesis. The 
      present study was designed to detect hTERC amplification in cervical lesions and 
      evaluate whether this might serve as a supportive biomarker to cytopathology or 
      histopathology in the diagnosis of cervical lesions. METHODS: Liquid-based 
      thin-layer cytopathologic examination and detection of amplification by 
      fluorescence in situ hybridization (FISH) was conducted in 130 women, along with 
      assessment of human papillomavirus DNA, colposcopy with biopsy, and 
      histopathologic examination. RESULTS: In cytopathologic examinations, hTERC 
      amplification rates for negative for intraepithelial lesion or malignancy 
      (NILM),atypical squamous cells of undetermined significance (ASCUS), low-grade 
      squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial 
      lesion (HSIL) and squamous cell carcinoma (SCC) cases were 0% (0/10), 4% (1/25), 
      20% (6/30), 77% (27/35), and 100% (10/10), respectively. The difference among 
      abnormal cellular change groups was statistically significant (P< 0.05). In 
      histopathologic examinations, hTERC amplification rates in normal squamous cell 
      with or without inflammatory, cervical intraepithelial neoplasia 1 (CIN 1), CIN 
      2, CIN 3 and SCC cases were 3.8% (2/52), 18.2% (6/33), 66.7% (6/9), 84.6% 
      (22/26), 100% (10/10), respectively. There were significant differences among 
      CIN1, CIN2, CIN3 and SCC cases (P< 0.05). The hTERC amplification was more 
      specific than HPV positivity in differentiating lowgrade from high-grade cervical 
      disorders (specificity: 88.5% vs. 70.8%, P< 0.05). CONCLUSIONS: FISH detection of 
      hTERC amplification could be an effective adjunct to cytopathologic or 
      histopathologic examination for differential diagnosis of low- and high-grade 
      cervical squamous cell disorders.
FAU - Jin, Yi
AU  - Jin Y
AD  - Department of Pathology, The Third Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou, China.
FAU - Li, Jia-Ping
AU  - Li JP
FAU - He, Dan
AU  - He D
FAU - Tang, Lu-Ying
AU  - Tang LY
FAU - Zee, Chi-shing
AU  - Zee CS
FAU - Guo, Shao-Zhong
AU  - Guo SZ
FAU - Zhou, Jing
AU  - Zhou J
FAU - Chen, Jian-Ning
AU  - Chen JN
FAU - Shao, Chun-Kui
AU  - Shao CK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Thailand
TA  - Asian Pac J Cancer Prev
JT  - Asian Pacific journal of cancer prevention : APJCP
JID - 101130625
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (DNA, Viral)
RN  - 0 (telomerase RNA)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Adult
MH  - Alphapapillomavirus/genetics/isolation & purification
MH  - Biomarkers, Tumor/genetics
MH  - DNA, Viral/analysis
MH  - Female
MH  - *Gene Amplification
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Neoplasms, Squamous Cell/*genetics
MH  - Papillomavirus Infections
MH  - RNA/*genetics
MH  - Telomerase/*genetics
MH  - Uterine Cervical Diseases/diagnosis/genetics/pathology
MH  - Uterine Cervical Neoplasms/diagnosis/*genetics/pathology
MH  - Uterine Cervical Dysplasia/*genetics
EDAT- 2011/08/31 06:00
MHDA- 2012/05/30 06:00
CRDT- 2011/08/31 06:00
PHST- 2011/08/31 06:00 [entrez]
PHST- 2011/08/31 06:00 [pubmed]
PHST- 2012/05/30 06:00 [medline]
PST - ppublish
SO  - Asian Pac J Cancer Prev. 2011;12(5):1167-71.