PMID- 21875394
OWN - NLM
STAT- MEDLINE
DCOM- 20120925
LR  - 20161018
IS  - 1437-4331 (Electronic)
IS  - 1434-6621 (Linking)
VI  - 49
IP  - 12
DP  - 2011 Aug 29
TI  - Rapid and simple IgG specific test for the exclusion of heparin induced 
      thrombocytopenia (HIT).
PG  - 2065-8
LID - /j/cclm.2011.49.issue-12/cclm.2011.693/cclm.2011.693.xml [pii]
LID - 10.1515/CCLM.2011.693 [doi]
AB  - BACKGROUND: The exclusion of heparin induced thrombocytopenia (HIT) is required 
      for selecting the most appropriate anticoagulation therapy in affected patients. 
      It requires the combination of clinical data with the detection of antibodies 
      directed against platelet factor 4 (PF4) in complex with polyanions (PA) such as 
      heparin. METHODS: We developed a lateral flow immunoassay (LFIA) for PF4/PA 
      complex specific IgG antibodies based on gold nanoparticles. Unlike most other 
      assays, the initial immune reaction takes place in the liquid phase. The 
      sensitivity of the assay has been adjusted with clinical samples aiming in the 
      reliable detection of sera which are positive in a functional platelet activation 
      assay. RESULTS: Sera from 60 patients with suspected HIT were investigated. LFIA 
      identified correctly all samples (n=20) which were positive in a functional assay 
      (HIPA) and an IgG specific ELISA. It correlated with ELISA, but false positive 
      results were less frequent (7 samples were negative with LFIA and HIPA but 
      positive with ELISA). CONCLUSIONS: The LFIA may be a suitable tool for the rapid 
      exclusion of HIT within 10 min.
FAU - Kolde, Hans-Jurgen
AU  - Kolde HJ
AD  - Consulting Diagnostics, Ottobrunn, Germany. h.-J.kolde@t-online.de
FAU - Dostatni, Ralf
AU  - Dostatni R
FAU - Mauracher, Susanne
AU  - Mauracher S
LA  - eng
PT  - Journal Article
DEP - 20110829
PL  - Germany
TA  - Clin Chem Lab Med
JT  - Clinical chemistry and laboratory medicine
JID - 9806306
RN  - 0 (Immunoglobulin G)
RN  - 37270-94-3 (Platelet Factor 4)
RN  - 7440-57-5 (Gold)
RN  - 9005-49-6 (Heparin)
SB  - IM
MH  - Gold/chemistry
MH  - Heparin/*adverse effects
MH  - Humans
MH  - *Immunoassay
MH  - Immunoglobulin G/*blood
MH  - Metal Nanoparticles/chemistry
MH  - Platelet Factor 4/immunology
MH  - Thrombocytopenia/*blood/chemically induced/diagnosis
EDAT- 2011/08/31 06:00
MHDA- 2012/09/26 06:00
CRDT- 2011/08/31 06:00
PHST- 2011/07/27 00:00 [received]
PHST- 2011/08/31 06:00 [entrez]
PHST- 2011/08/31 06:00 [pubmed]
PHST- 2012/09/26 06:00 [medline]
AID - /j/cclm.2011.49.issue-12/cclm.2011.693/cclm.2011.693.xml [pii]
AID - 10.1515/CCLM.2011.693 [doi]
PST - epublish
SO  - Clin Chem Lab Med. 2011 Aug 29;49(12):2065-8. doi: 10.1515/CCLM.2011.693.