PMID- 21875545
OWN - NLM
STAT- MEDLINE
DCOM- 20111027
LR  - 20110830
IS  - 1534-4436 (Electronic)
IS  - 1081-1206 (Linking)
VI  - 107
IP  - 3
DP  - 2011 Sep
TI  - Glucocorticoid-induced immunoglobulin E synthesis by peripheral blood mononuclear 
      cells from allergic and nonallergic subjects.
PG  - 251-7
LID - 10.1016/j.anai.2011.05.028 [doi]
AB  - BACKGROUND: Glucocorticoids (GCS) have been shown to induce IgE synthesis in 
      human peripheral blood mononuclear cells (PBMCs) and purified B cells in vitro. 
      However, the differences in immunoglobulin E (IgE) response to GCS between 
      allergic and non-allergic individuals and the mechanism this interaction have not 
      been elucidated. OBJECTIVE: We aimed to compare the effect of GCS (budesonide) on 
      interleukin (IL)-4-driven IgE production in vitro in allergic and non allergic 
      subjects and assess the engagement of intracellular mechanisms. METHODS: The 
      study included 22 patients with allergic asthma and/or allergic rhinitis and 24 
      healthy volunteers. PBMCs were cultured for 11 days with IL-4 and budesonide and 
      IgE concentrations in supernatants were assessed by immunoassays. T and B cell 
      markers were assessed by flow cytometry. RESULTS: Budesonide enhanced IgE 
      synthesis to higher extent in healthy donors than in allergic patients (mean 
      increase of 16.5 vs 6.3 kU/L, P< .05 respectively) acting through glucocorticoid 
      receptor. Budesonide significantly increased lymhoplasmocytoid cells percentage 
      in both media-controlled (2.5-fold increase) and IL-4-stimulated PBMCs (2-fold 
      increase). Added to IL-4 budesonide decreased the percentage of both T cells and 
      CD40L(+) T cells, but strongly increased the percentage of B cells. Protein 
      tyrosine kinase (PTK) inhibitor decreased, but NF-kappaB and protein kinase A (PKA) 
      inhibitors expressed modulatory effects on budesonide-induced IgE synthesis. 
      CONCLUSIONS: Budesonide-induced IgE generation in PBMCs differs in magnitude and 
      seems to involve different mechanisms in atopic and non-atopic subjects.
CI  - Copyright (c) 2011 American College of Allergy, Asthma & Immunology. Published by 
      Elsevier Inc. All rights reserved.
FAU - Chalubinski, Maciej
AU  - Chalubinski M
AD  - Department of Immunology, Rheumatology and Allergy, Faculty of Medicine, Medical 
      University of Lodz, Lodz, Poland.
FAU - Grzegorczyk, Janina
AU  - Grzegorczyk J
FAU - Kowalski, Marek L
AU  - Kowalski ML
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110722
PL  - United States
TA  - Ann Allergy Asthma Immunol
JT  - Annals of allergy, asthma & immunology : official publication of the American 
      College of Allergy, Asthma, & Immunology
JID - 9503580
RN  - 0 (Glucocorticoids)
RN  - 0 (NF-kappa B)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 147205-72-9 (CD40 Ligand)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 51333-22-3 (Budesonide)
SB  - IM
MH  - Adult
MH  - Asthma/*immunology
MH  - B-Lymphocytes/drug effects/immunology
MH  - Budesonide/pharmacology
MH  - CD40 Ligand/immunology
MH  - Female
MH  - Glucocorticoids/*pharmacology
MH  - Humans
MH  - Hypersensitivity/*immunology
MH  - Immunoglobulin E/*biosynthesis
MH  - Interleukin-4/immunology
MH  - Leukocytes, Mononuclear/*drug effects/immunology
MH  - Male
MH  - Middle Aged
MH  - NF-kappa B/antagonists & inhibitors/immunology
MH  - Protein Kinase Inhibitors/pharmacology
MH  - Rhinitis, Allergic, Perennial/*immunology
MH  - T-Lymphocytes/drug effects/immunology
MH  - Young Adult
EDAT- 2011/08/31 06:00
MHDA- 2011/10/28 06:00
CRDT- 2011/08/31 06:00
PHST- 2011/02/01 00:00 [received]
PHST- 2011/05/24 00:00 [revised]
PHST- 2011/05/31 00:00 [accepted]
PHST- 2011/08/31 06:00 [entrez]
PHST- 2011/08/31 06:00 [pubmed]
PHST- 2011/10/28 06:00 [medline]
AID - S1081-1206(11)00420-0 [pii]
AID - 10.1016/j.anai.2011.05.028 [doi]
PST - ppublish
SO  - Ann Allergy Asthma Immunol. 2011 Sep;107(3):251-7. doi: 
      10.1016/j.anai.2011.05.028. Epub 2011 Jul 22.