PMID- 2187807
OWN - NLM
STAT- MEDLINE
DCOM- 19900625
LR  - 20210526
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 58
IP  - 6
DP  - 1990 Jun
TI  - Demonstration of cross-reactivity between bacterial antigens and class I human 
      leukocyte antigens by using monoclonal antibodies to Shigella flexneri.
PG  - 1774-81
AB  - Bacterial envelope proteins which share immunodeterminants with the human 
      leukocyte antigen (HLA) class I histocompatibility antigen HLA-B27 may invoke 
      spondyloarthritic disease through the process of molecular mimicry in patients 
      expressing this phenotype. Monoclonal antibodies generated by the immunization of 
      BALB/c mice with envelope proteins of Shigella flexneri type 2a were tested for 
      reactivity against cultured lymphoblastoid cell lines of defined HLA phenotype. 
      As measured by flow microfluorometry, four immunoglobulin M monoclonal antibodies 
      reacted preferentially with HLA-B27-positive lymphocytes (HOM-2, MM) as compared 
      with a B27-loss mutant line (1065) or cells lacking major histocompatibility 
      complex class I antigen (Daudi, K562). Monoclonal antibodies also reacted with 
      mouse EL-4 cells transfected with and expressing the HLA-B7 gene. Western 
      immunoblot analysis of isolated enterobacterial envelopes demonstrated that the 
      reactive epitope was present on bacterial proteins with an apparent relative 
      molecular mass of 36 and 19 kilodaltons. The structural basis for the 
      cross-reactivity of bacterial antigen and HLA-B27 appeared to reside in the 
      portion of the HLA molecule that is responsible for allotypic specificity (amino 
      acids 63 through 83), since monoclonal antibodies were positive by enzyme-linked 
      immunosorbent assay with synthetic polypeptides corresponding to this segment.
FAU - Williams, K M
AU  - Williams KM
AD  - Division of Microbiology, Food and Drug Administration, Washington, D.C. 20204.
FAU - Raybourne, R B
AU  - Raybourne RB
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Proteins)
RN  - 0 (HLA-B Antigens)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antigens, Bacterial/*immunology
MH  - Bacterial Proteins/immunology
MH  - Blotting, Western
MH  - Cross Reactions
MH  - HLA-B Antigens/*immunology
MH  - Humans
MH  - In Vitro Techniques
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Shigella flexneri/*immunology
PMC - PMC258722
EDAT- 1990/06/01 00:00
MHDA- 1990/06/01 00:01
PMCR- 1990/06/01
CRDT- 1990/06/01 00:00
PHST- 1990/06/01 00:00 [pubmed]
PHST- 1990/06/01 00:01 [medline]
PHST- 1990/06/01 00:00 [entrez]
PHST- 1990/06/01 00:00 [pmc-release]
AID - 10.1128/iai.58.6.1774-1781.1990 [doi]
PST - ppublish
SO  - Infect Immun. 1990 Jun;58(6):1774-81. doi: 10.1128/iai.58.6.1774-1781.1990.