PMID- 21879802
OWN - NLM
STAT- MEDLINE
DCOM- 20120227
LR  - 20220311
IS  - 1502-7708 (Electronic)
IS  - 0036-5521 (Linking)
VI  - 46
IP  - 11
DP  - 2011 Nov
TI  - Human herpesvirus 6 and cytomegalovirus in ileocolonic mucosa in inflammatory 
      bowel disease.
PG  - 1324-33
LID - 10.3109/00365521.2011.605466 [doi]
AB  - OBJECTIVES: Reactivation of a latent cytomegalovirus (CMV) may occur in 
      inflammatory bowel disease (IBD). Data of human herpesvirus 6 (HHV-6)--a close 
      relative to CMV--in active IBD are scarce. The aim of this study was to detect 
      HHV-6 and CMV antigens in the mucosa of active and inactive IBD. MATERIAL AND 
      METHODS: 79 IBD patients (47 ulcerative colitis (UC) and 32 Crohn's disease (CD)) 
      were recruited and endoscopic and histological disease activity was scored. 
      Control group consisted of 15 non-IBD patients with normal colonoscopy. 
      Immunohistochemical stainings for HHV-6B and CMV antigens were performed on 
      biopsy specimens from the ileum and colorectum. The intensity of HHV-6B and CMV 
      expression was graded as negative, mild, moderate, or intense. RESULTS: HHV-6B 
      antigen was positive in 35 (44%) and CMV in 64 (81%). Of controls, 6 (40%) were 
      mildly positive for HHV-6 and 6 (40%) for CMV. In IBD, both CMV and HHV-6B 
      intensity correlated with endoscopic disease severity (CMV p = 0.010 and HHV-6 p 
      = 0.048). Simultaneous HHV-6B and CMV antigen expression occurred in 29 (37%) and 
      associated with endoscopic activity (p = 0.006) and to a number of 
      immunosuppressives (p = 0.033). A significant difference in HHV-6B positivity was 
      found between endoscopically active and inactive UC (p = 0.040). Both CMV and 
      HHV-6B intensity correlated with histological severity in the rectal biopsy 
      specimens (for CMV p = 0.040 and for HHV-6B p = 0.027). CONCLUSIONS: Both viruses 
      occurred ubiquitously in the IBD mucosa. Coexistence of viruses was common and 
      associated with disease activity and use of immunosuppressives. HHV-6B intensity 
      correlated with endoscopic severity in UC.
FAU - Sipponen, Taina
AU  - Sipponen T
AD  - Department of Medicine, Division of Gastroenterology, Helsinki University Central 
      Hospital, Helsinki, Finland. taina.sipponen@hus.fi
FAU - Turunen, Ulla
AU  - Turunen U
FAU - Lautenschlager, Irmeli
AU  - Lautenschlager I
FAU - Nieminen, Urpo
AU  - Nieminen U
FAU - Arola, Johanna
AU  - Arola J
FAU - Halme, Leena
AU  - Halme L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110831
PL  - England
TA  - Scand J Gastroenterol
JT  - Scandinavian journal of gastroenterology
JID - 0060105
RN  - 0 (Antigens, Viral)
RN  - 0 (Immunosuppressive Agents)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antigens, Viral/analysis
MH  - Colitis, Ulcerative/drug therapy/pathology/*virology
MH  - Colon/virology
MH  - Crohn Disease/drug therapy/pathology/*virology
MH  - Cytomegalovirus/*immunology/isolation & purification
MH  - Cytomegalovirus Infections/complications/*immunology
MH  - Female
MH  - Herpesviridae Infections/complications/*immunology
MH  - Herpesvirus 6, Human/*immunology/isolation & purification
MH  - Humans
MH  - Ileum/virology
MH  - Immunocompromised Host/immunology
MH  - Immunohistochemistry
MH  - Immunosuppressive Agents/adverse effects/therapeutic use
MH  - Intestinal Mucosa/*virology
MH  - Male
MH  - Middle Aged
MH  - Prospective Studies
MH  - Severity of Illness Index
MH  - Young Adult
EDAT- 2011/09/02 06:00
MHDA- 2012/03/01 06:00
CRDT- 2011/09/02 06:00
PHST- 2011/09/02 06:00 [entrez]
PHST- 2011/09/02 06:00 [pubmed]
PHST- 2012/03/01 06:00 [medline]
AID - 10.3109/00365521.2011.605466 [doi]
PST - ppublish
SO  - Scand J Gastroenterol. 2011 Nov;46(11):1324-33. doi: 
      10.3109/00365521.2011.605466. Epub 2011 Aug 31.