PMID- 21883278
OWN - NLM
STAT- MEDLINE
DCOM- 20120222
LR  - 20211020
IS  - 1398-9995 (Electronic)
IS  - 0105-4538 (Print)
IS  - 0105-4538 (Linking)
VI  - 66
IP  - 12
DP  - 2011 Dec
TI  - Computationally predicted IgE epitopes of walnut allergens contribute to 
      cross-reactivity with peanuts.
PG  - 1522-9
LID - 10.1111/j.1398-9995.2011.02692.x [doi]
AB  - BACKGROUND: Cross-reactivity between peanuts and tree nuts implies that similar 
      immunoglobulin E (IgE) epitopes are present in their proteins. OBJECTIVE: To 
      determine whether walnut sequences similar to known peanut IgE-binding sequences, 
      according to the property distance (PD) scale implemented in the Structural 
      Database of Allergenic Proteins, react with IgE from sera of patients with 
      allergy to walnut and/or peanut. METHODS:   Patient sera were characterized by 
      western blotting for IgE binding to nut protein extracts and to peptides from 
      walnut and peanut allergens, similar to known peanut epitopes as defined by low 
      PD values, synthesized on membranes. Competitive enzyme-linked immunosorbent 
      assay (ELISA) was used to show that peanut and predicted walnut epitope sequences 
      compete with purified Ara h 2 for binding to IgE in serum from a cross-reactive 
      patient. RESULTS: Sequences from the vicilin walnut allergen Jug r 2, which had 
      low PD values to epitopes of the peanut allergen Ara h 2, a 2S albumin, bound to 
      IgE in sera from five patients who reacted to either walnut or peanut or both. A 
      walnut epitope recognized by sera from six patients mapped to a surface-exposed 
      region on a model of the N-terminal pro-region of Jug r 2. This predicted walnut 
      epitope competed for IgE binding to Ara h 2 in serum as well as the known IgE 
      epitope from Ara h 2. CONCLUSIONS: Sequences with low PD value (< 8.5) to known 
      IgE epitopes could contribute to cross-reactivity between allergens. This further 
      validates the PD scoring method for predicting cross-reactive epitopes in 
      allergens.
CI  - (c) 2011 John Wiley & Sons A/S.
FAU - Maleki, S J
AU  - Maleki SJ
AD  - U.S. Department of Agriculture-Agricultural Research Service-Southern Regional 
      Research Center (USDA-ARS-SRRC), New Orleans, LA 70124, USA. 
      soheila.maleki@ars.usda.gov
FAU - Teuber, S S
AU  - Teuber SS
FAU - Cheng, H
AU  - Cheng H
FAU - Chen, D
AU  - Chen D
FAU - Comstock, S S
AU  - Comstock SS
FAU - Ruan, S
AU  - Ruan S
FAU - Schein, C H
AU  - Schein CH
LA  - eng
GR  - R01 AI064913/AI/NIAID NIH HHS/United States
GR  - R01 AI064913-05S1/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20110823
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Allergens)
RN  - 0 (Epitopes)
RN  - 0 (Plant Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*immunology/metabolism
MH  - Amino Acid Sequence
MH  - Arachis/*immunology
MH  - Binding, Competitive/immunology
MH  - Child, Preschool
MH  - Cross Reactions/immunology
MH  - Databases, Protein
MH  - Epitopes/chemistry/*immunology
MH  - Food Hypersensitivity/immunology
MH  - Humans
MH  - Immunoglobulin E/*immunology/metabolism
MH  - Infant
MH  - Juglans/*immunology
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Plant Proteins/immunology/metabolism
MH  - Protein Binding/immunology
MH  - Protein Conformation
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
PMC - PMC3203311
MID - NIHMS314549
COIS- Conflict of interest statement: None of the authors have any conflict of 
      interests to disclose regarding this manuscript.
EDAT- 2011/09/03 06:00
MHDA- 2012/02/23 06:00
PMCR- 2012/12/01
CRDT- 2011/09/03 06:00
PHST- 2011/09/03 06:00 [entrez]
PHST- 2011/09/03 06:00 [pubmed]
PHST- 2012/02/23 06:00 [medline]
PHST- 2012/12/01 00:00 [pmc-release]
AID - 10.1111/j.1398-9995.2011.02692.x [doi]
PST - ppublish
SO  - Allergy. 2011 Dec;66(12):1522-9. doi: 10.1111/j.1398-9995.2011.02692.x. Epub 2011 
      Aug 23.