PMID- 21883550
OWN - NLM
STAT- MEDLINE
DCOM- 20120504
LR  - 20221207
IS  - 1365-313X (Electronic)
IS  - 0960-7412 (Linking)
VI  - 68
IP  - 6
DP  - 2011 Dec
TI  - Chromosomal rearrangements between tomato and Solanum chilense hamper mapping and 
      breeding of the TYLCV resistance gene Ty-1.
PG  - 1093-103
LID - 10.1111/j.1365-313X.2011.04762.x [doi]
AB  - Tomato yellow leaf curl disease, a devastating disease of Solanum lycopersicum 
      (tomato), is caused by a complex of begomoviruses generally referred to as Tomato 
      yellow leaf curl virus (TYLCV). Almost all breeding for TYLCV resistance has been 
      based on the introgression of the Ty-1 resistance locus derived from Solanum 
      chilense LA1969. Knowledge about the exact location of Ty-1 on tomato chromosome 
      6 will help in understanding the genomic organization of the Ty-1 locus. In this 
      study, we analyze the chromosomal rearrangement and recombination behavior of the 
      chromosomal region where Ty-1 is introgressed. Nineteen markers on tomato 
      chromosome 6 were used in F(2) populations obtained from two commercial hybrids, 
      and showed the presence of a large introgression in both. Fluorescence in situ 
      hybridization (FISH) analysis revealed two chromosomal rearrangements between S. 
      lycopersicum and S. chilense LA1969 in the Ty-1 introgression. Furthermore, a 
      large-scale recombinant screening in the two F(2) populations was performed, and 
      30 recombinants in the Ty-1 introgression were identified. All recombination 
      events were located on the long arm beyond the inversions, showing that 
      recombination in the inverted region was absent. Disease tests on progenies of 
      informative recombinants with TYLCV mapped Ty-1 to the long arm between markers 
      MSc05732-4 and MSc05732-14, an interval overlapping with the reported Ty-3 
      region, which led to the indication that Ty-1 and Ty-3 may be allelic. With this 
      study we prove that FISH can be used as a diagnostic tool to aid in the accurate 
      mapping of genes that were introgressed from wild species into cultivated tomato.
CI  - (c) 2011 The Authors. The Plant Journal (c) 2011 Blackwell Publishing Ltd.
FAU - Verlaan, Maarten G
AU  - Verlaan MG
AD  - Wageningen UR Plant Breeding, Wageningen University, Droevendaalsesteeg 1, 6708 
      PB Wageningen, the Netherlands.
FAU - Szinay, Dora
AU  - Szinay D
FAU - Hutton, Samuel F
AU  - Hutton SF
FAU - de Jong, Hans
AU  - de Jong H
FAU - Kormelink, Richard
AU  - Kormelink R
FAU - Visser, Richard G F
AU  - Visser RG
FAU - Scott, John W
AU  - Scott JW
FAU - Bai, Yuling
AU  - Bai Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20111013
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
SB  - IM
MH  - Begomovirus/*genetics
MH  - Chromosome Mapping/methods
MH  - Chromosomes, Plant/*genetics
MH  - Disease Resistance/*genetics
MH  - *Gene Rearrangement
MH  - *Genes, Plant
MH  - In Situ Hybridization, Fluorescence
MH  - Solanum lycopersicum/genetics/virology
MH  - Plant Diseases/*genetics
MH  - Recombination, Genetic
MH  - Solanum/*genetics/virology
EDAT- 2011/09/03 06:00
MHDA- 2012/05/05 06:00
CRDT- 2011/09/03 06:00
PHST- 2011/09/03 06:00 [entrez]
PHST- 2011/09/03 06:00 [pubmed]
PHST- 2012/05/05 06:00 [medline]
AID - 10.1111/j.1365-313X.2011.04762.x [doi]
PST - ppublish
SO  - Plant J. 2011 Dec;68(6):1093-103. doi: 10.1111/j.1365-313X.2011.04762.x. Epub 
      2011 Oct 13.