PMID- 21884716
OWN - NLM
STAT- MEDLINE
DCOM- 20120113
LR  - 20211020
IS  - 1096-0333 (Electronic)
IS  - 0041-008X (Print)
IS  - 0041-008X (Linking)
VI  - 257
IP  - 1
DP  - 2011 Nov 15
TI  - Organic anion transporting polypeptides in the hepatic uptake of PBDE congeners 
      in mice.
PG  - 23-31
LID - 10.1016/j.taap.2011.08.014 [doi]
AB  - BDE47, BDE99 and BDE153 are the predominant polybrominated diphenyl ether (PBDE) 
      congeners detected in humans and can induce drug metabolizing enzymes in the 
      liver. We have previously demonstrated that several human liver organic anion 
      transporting polypeptides (humans: OATPs; rodents: Oatps) can transport PBDE 
      congeners. Mice are commonly used to study the toxicity of chemicals like the 
      PBDE congeners. However, the mechanism of the hepatic PBDE uptake in mice is not 
      known. Therefore, the purpose of the current study was to test the hypothesis 
      that BDE47, BDE99, and BDE153 are substrates of mouse hepatic Oatps (Oatp1a1, 
      Oatp1a4, Oatp1b2, and Oatp2b1). We used Human Embryonic Kidney 293 (HEK293) cells 
      transiently expressing individual Oatps and quantified the uptake of BDE47, 
      BDE99, and BDE153. Oatp1a4, Oatp1b2, and Oatp2b1 transported all three PBDE 
      congeners, whereas Oatp1a1 did transport none. Kinetic studies demonstrated that 
      Oatp1a4 and Oatp1b2 transported BDE47 with the greatest affinity, followed by 
      BDE99 and BDE153. In contrast, Oatp2b1 transported all three PBDE congeners with 
      similar affinities. The importance of hepatic Oatps for the liver accumulation of 
      BDE47 was confirmed using Oatp1a4-, and Oatp1b2-null mice.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Pacyniak, Erik
AU  - Pacyniak E
AD  - Department of Pharmacology, Toxicology and Therapeutics, University of Kansas 
      Medical Center, Kansas City, KS 66160, USA.
FAU - Hagenbuch, Bruno
AU  - Hagenbuch B
FAU - Klaassen, Curtis D
AU  - Klaassen CD
FAU - Lehman-McKeeman, Lois
AU  - Lehman-McKeeman L
FAU - Guo, Grace L
AU  - Guo GL
LA  - eng
GR  - RR021940/RR/NCRR NIH HHS/United States
GR  - GM077336/GM/NIGMS NIH HHS/United States
GR  - P20 RR021940/RR/NCRR NIH HHS/United States
GR  - R01 GM077336/GM/NIGMS NIH HHS/United States
GR  - DK081343/DK/NIDDK NIH HHS/United States
GR  - R01 DK081343-04/DK/NIDDK NIH HHS/United States
GR  - R01 DK081343/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20110822
PL  - United States
TA  - Toxicol Appl Pharmacol
JT  - Toxicology and applied pharmacology
JID - 0416575
RN  - 0 (2,2',4,4',5-brominated diphenyl ether)
RN  - 0 (Halogenated Diphenyl Ethers)
RN  - 0 (Organic Anion Transport Protein 1)
RN  - 0 (Organic Anion Transporters)
RN  - 0 (Polybrominated Biphenyls)
RN  - 0 (hexabrominated diphenyl ether 153)
RN  - 0N97R5X10X (2,2',4,4'-tetrabromodiphenyl ether)
SB  - IM
MH  - Animals
MH  - Cloning, Molecular
MH  - Dose-Response Relationship, Drug
MH  - HEK293 Cells/metabolism
MH  - Halogenated Diphenyl Ethers/*metabolism
MH  - Humans
MH  - Liver/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Organic Anion Transport Protein 1/metabolism
MH  - Organic Anion Transporters/*metabolism
MH  - Polybrominated Biphenyls/metabolism
PMC - PMC3220748
MID - NIHMS325135
EDAT- 2011/09/03 06:00
MHDA- 2012/01/14 06:00
PMCR- 2012/11/15
CRDT- 2011/09/03 06:00
PHST- 2011/05/17 00:00 [received]
PHST- 2011/08/08 00:00 [revised]
PHST- 2011/08/13 00:00 [accepted]
PHST- 2011/09/03 06:00 [entrez]
PHST- 2011/09/03 06:00 [pubmed]
PHST- 2012/01/14 06:00 [medline]
PHST- 2012/11/15 00:00 [pmc-release]
AID - S0041-008X(11)00312-7 [pii]
AID - 10.1016/j.taap.2011.08.014 [doi]
PST - ppublish
SO  - Toxicol Appl Pharmacol. 2011 Nov 15;257(1):23-31. doi: 
      10.1016/j.taap.2011.08.014. Epub 2011 Aug 22.