PMID- 21885037
OWN - NLM
STAT- MEDLINE
DCOM- 20120127
LR  - 20171116
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1416
DP  - 2011 Oct 6
TI  - RACK1 affects morphine reward via BDNF.
PG  - 26-34
LID - 10.1016/j.brainres.2011.07.045 [doi]
AB  - Chronic morphine addiction may trigger functional changes in the mesolimbic 
      dopamine system, which is believed to be the neurobiological substrate of opiate 
      addiction. Brain derived neurotrophic factor (BDNF) has been implicated in 
      addiction-related pathology in animal studies. Our previous studies have shown 
      that RACK1 is involved in morphine reward in mice. The recent research indicates 
      nuclear RACK1 by localizing at the promoter IV region of the BDNF gene and the 
      subsequent chromatin modifications leads to the activation of the promoter and 
      transcription of BDNF. The present study was designed to investigate if shRACK1 
      (a short hairpin RNA of RACK1) could reverse the mice's behavioral responses to 
      morphine and BDNF expression in hippocampus and prefrontal cortex. No significant 
      changes were observed in vehicle-infused mice which received no morphine 
      treatment (CONC) and shRACK1-infused mice which received no morphine treatment 
      (CONR), whereas vehicle-infused mice preceded the morphine injection (MIC) showed 
      increased BDNF expression in hippocampus and prefrontal cortex, as compared to 
      vehicle-infused mice which received no morphine treatment (CONC). 
      Intracerebroventricular shRACK1 treatment reversed these, and in fact, 
      ShRACK1-infused mice preceded the morphine injection (MIR) showed reduced BDNF 
      expression in hippocampus and prefrontal cortex, as compared to MIC. In the 
      conditioned place preference (CPP) test, inactivating RACK1 markedly reduces 
      morphine-induced conditioned place preference. Non-specific changes in CPP could 
      not account for these effects since general CPP of shRACK1- and vehicle-infused 
      animals was not different. Combined behavioral and molecular approaches have 
      support the possibility that the RACK1-BDNF system plays an important role in the 
      response to morphine-induced reward.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Wan, Lihong
AU  - Wan L
AD  - Key Laboratory of Chronobiology, Ministry of Health (Sichuan University), Sichuan 
      University, Chengdu, PR China. bigeyes812@163.com
FAU - Xie, Yizhou
AU  - Xie Y
FAU - Su, Lan
AU  - Su L
FAU - Liu, Yanyou
AU  - Liu Y
FAU - Wang, Yuhui
AU  - Wang Y
FAU - Wang, Zhengrong
AU  - Wang Z
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110728
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Narcotics)
RN  - 0 (Neuropeptides)
RN  - 0 (Peptide Fragments)
RN  - 0 (RACK1 protein, mouse)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Receptors for Activated C Kinase)
RN  - 76I7G6D29C (Morphine)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Association Learning/drug effects/*physiology
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Gene Expression Regulation/*physiology
MH  - Hippocampus/drug effects/*metabolism
MH  - Injections, Intraventricular
MH  - Inverted Repeat Sequences
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Morphine/pharmacology
MH  - Narcotics/pharmacology
MH  - Neuropeptides/genetics/*metabolism
MH  - Peptide Fragments
MH  - Prefrontal Cortex/drug effects/*metabolism
MH  - RNA, Small Interfering
MH  - Random Allocation
MH  - Receptors for Activated C Kinase
MH  - Reward
EDAT- 2011/09/03 06:00
MHDA- 2012/01/28 06:00
CRDT- 2011/09/03 06:00
PHST- 2011/06/09 00:00 [received]
PHST- 2011/07/21 00:00 [revised]
PHST- 2011/07/22 00:00 [accepted]
PHST- 2011/09/03 06:00 [entrez]
PHST- 2011/09/03 06:00 [pubmed]
PHST- 2012/01/28 06:00 [medline]
AID - S0006-8993(11)01373-4 [pii]
AID - 10.1016/j.brainres.2011.07.045 [doi]
PST - ppublish
SO  - Brain Res. 2011 Oct 6;1416:26-34. doi: 10.1016/j.brainres.2011.07.045. Epub 2011 
      Jul 28.