PMID- 21891822
OWN - NLM
STAT- MEDLINE
DCOM- 20120221
LR  - 20121115
IS  - 1735-1502 (Print)
IS  - 1735-1502 (Linking)
VI  - 10
IP  - 3
DP  - 2011 Sep
TI  - Spleen and liver dendritic cells differ in their tolerogenic and cytokine 
      induction potential.
PG  - 163-70
AB  - Dendritic cells (DCs) play an important role in induction of cellular immune 
      responses. It seems that DCs that reside in different organs may be distinct in 
      their ability to induce immune responses. This study was done to address the 
      differences between spleen and liver DCs in induction of immune response and/or 
      tolerance. CD11c+ DCs were separated from the liver and spleen of C57BL/6 mice 
      and pulsed with myelin oligodendrocyte glycoprotein (MOG) peptide 35-55. 6105 
      MOG35-55 pulsed spleen or liver DCs were injected in foot pad of different groups 
      of mice. Control groups received unpulsed DCs. After 5 days, the mononuclear 
      cells (MNCs) of the regional lymph nodes were isolated from immunized mice for 
      cytokine assays and lymphocyte transformation test. To study the immunologic or 
      tolerogenic effects of DCs, three weeks after immunization of mice with MOG 
      pulsed liver or spleen DCs, experimental autoimmune encephalomyelitis (EAE) was 
      induced in DC-immunized mice by injection of MOG along with complete Freund's 
      adjuvant. Our results showed that spleen DCs were more potent in stimulating 
      lymph node T cells as illustrated in lymphocyte transformation test. Moreover 
      IL-10 production was higher in mice immunized with liver DCs compared with those 
      immunized with splenic DCs (p=0.017). However, no significant difference in IFN-gamma 
      production was observed between two groups. We also found that liver DCs+MOG 
      immunized mice displayed a significantly delayed disease onset compared with 
      spleen DCs+MOG immunized mice and the control groups. The disease score was also 
      milder in liver DCs immunized mice compared with other groups. It seems that the 
      higher IL-10 production induced by the liver DCs may be one of the main factors 
      in down regulation of immune responses in this organ. It can be concluded also 
      that the liver DCs may inhibit the progress of EAE by shifting the cytokines 
      profile.
FAU - Mosayebi, Ghasem
AU  - Mosayebi G
AD  - Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares 
      University, Tehran, Iran.
FAU - Moazzeni, Seyed Mohammad
AU  - Moazzeni SM
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Allergy Asthma Immunol
JT  - Iranian journal of allergy, asthma, and immunology
JID - 101146178
RN  - 0 (Cytokines)
RN  - 0 (Glycoproteins)
RN  - 0 (Myelin-Oligodendrocyte Glycoprotein)
RN  - 0 (Peptide Fragments)
RN  - 0 (myelin oligodendrocyte glycoprotein (35-55))
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - Cytokines/*biosynthesis/immunology
MH  - Dendritic Cells/*immunology
MH  - Encephalomyelitis, Autoimmune, Experimental/immunology
MH  - Female
MH  - Glycoproteins/immunology
MH  - Immune Tolerance/*immunology
MH  - Interleukin-10/biosynthesis/immunology
MH  - Liver/cytology/*immunology
MH  - Lymphocyte Activation/*immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Myelin-Oligodendrocyte Glycoprotein
MH  - Peptide Fragments/immunology
MH  - Spleen/cytology/*immunology
EDAT- 2011/09/06 06:00
MHDA- 2012/02/22 06:00
CRDT- 2011/09/06 06:00
PHST- 2011/09/06 06:00 [entrez]
PHST- 2011/09/06 06:00 [pubmed]
PHST- 2012/02/22 06:00 [medline]
AID - 01003163170 [pii]
PST - ppublish
SO  - Iran J Allergy Asthma Immunol. 2011 Sep;10(3):163-70.