PMID- 21892953 OWN - NLM STAT- MEDLINE DCOM- 20111223 LR - 20240117 IS - 1532-429X (Electronic) IS - 1097-6647 (Print) IS - 1097-6647 (Linking) VI - 13 IP - 1 DP - 2011 Sep 5 TI - Rapid assessment of myocardial infarct size in rodents using multi-slice inversion recovery late gadolinium enhancement CMR at 9.4T. PG - 44 LID - 10.1186/1532-429X-13-44 [doi] AB - BACKGROUND: Myocardial infarction (MI) can be readily assessed using late gadolinium enhancement (LGE) cardiovascular magnetic resonance (CMR). Inversion recovery (IR) sequences provide the highest contrast between enhanced infarct areas and healthy myocardium. Applying such methods to small animals is challenging due to rapid respiratory and cardiac rates relative to T1 relaxation. METHODS: Here we present a fast and robust protocol for assessing LGE in small animals using a multi-slice IR gradient echo sequence for efficient assessment of LGE. An additional Look-Locker sequence was used to assess the optimum inversion point on an individual basis and to determine most appropriate gating points for both rat and mouse. The technique was applied to two preclinical scenarios: i) an acute (2 hour) reperfused model of MI in rats and ii) mice 2 days following non-reperfused MI. RESULTS: LGE images from all animals revealed clear areas of enhancement allowing for easy volume segmentation. Typical inversion times required to null healthy myocardium in rats were between 300-450 ms equivalent to 2-3 R-waves and ~330 ms in mice, typically 3 R-waves following inversion. Data from rats was also validated against triphenyltetrazolium chloride staining and revealed close agreement for infarct size. CONCLUSION: The LGE protocol presented provides a reliable method for acquiring images of high contrast and quality without excessive scan times, enabling higher throughput in experimental studies requiring reliable assessment of MI. FAU - Price, Anthony N AU - Price AN AD - UCL Centre for Advanced Biomedical Imaging, Department of Medicine and UCL Institute of Child Health, University College London, Medical School, and The Hatter Cardiovascular Institute, University College London Hospital, UK. a.price@ich.ucl.ac.uk FAU - Cheung, King K AU - Cheung KK FAU - Lim, Shiang Y AU - Lim SY FAU - Yellon, Derek M AU - Yellon DM FAU - Hausenloy, Derek J AU - Hausenloy DJ FAU - Lythgoe, Mark F AU - Lythgoe MF LA - eng GR - Biotechnology and Biological Sciences Research Council/United Kingdom GR - British Heart Foundation/United Kingdom PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20110905 PL - England TA - J Cardiovasc Magn Reson JT - Journal of cardiovascular magnetic resonance : official journal of the Society for Cardiovascular Magnetic Resonance JID - 9815616 RN - 0 (Contrast Media) RN - K2I13DR72L (Gadolinium DTPA) SB - IM MH - Animals MH - *Contrast Media MH - Disease Models, Animal MH - *Gadolinium DTPA MH - Image Interpretation, Computer-Assisted MH - *Magnetic Resonance Imaging, Cine MH - Male MH - Mice MH - Myocardial Infarction/*diagnosis MH - Myocardium/*pathology MH - Predictive Value of Tests MH - Rats MH - Rats, Wistar MH - Severity of Illness Index MH - Time Factors PMC - PMC3182945 EDAT- 2011/09/07 06:00 MHDA- 2011/12/24 06:00 PMCR- 2011/09/05 CRDT- 2011/09/07 06:00 PHST- 2011/04/12 00:00 [received] PHST- 2011/09/05 00:00 [accepted] PHST- 2011/09/07 06:00 [entrez] PHST- 2011/09/07 06:00 [pubmed] PHST- 2011/12/24 06:00 [medline] PHST- 2011/09/05 00:00 [pmc-release] AID - S1097-6647(23)01399-6 [pii] AID - 1532-429X-13-44 [pii] AID - 10.1186/1532-429X-13-44 [doi] PST - epublish SO - J Cardiovasc Magn Reson. 2011 Sep 5;13(1):44. doi: 10.1186/1532-429X-13-44.