PMID- 21893176
OWN - NLM
STAT- MEDLINE
DCOM- 20120118
LR  - 20161125
IS  - 1879-3169 (Electronic)
IS  - 0378-4274 (Linking)
VI  - 207
IP  - 1
DP  - 2011 Nov 10
TI  - In vitro and in ovo effects of four brominated flame retardants on toxicity and 
      hepatic mRNA expression in chicken embryos.
PG  - 25-33
LID - 10.1016/j.toxlet.2011.08.015 [doi]
AB  - Some currently used brominated flame retardants (BFRs), such as 
      hexachlorocyclopentadienyl-dibromocyclooctane (HCDBCO), 
      bis(2-ethylhexyl)tetrabromophthalate (BEHTBP), 
      1,2-bis(2,4,6-tribromophenoxy)ethane (BTBPE) and decabromodiphenylethane (DBDPE), 
      are persistent organic contaminants detected in various environmental matrices, 
      including wild birds. Data on potential toxicological and molecular responses to 
      exposure of these BFRs are lacking for avian species. A combined in vitro/in ovo 
      approach was used to determine the concentration-dependent effects of these BFRs 
      on overt toxicity and hepatic messenger RNA (mRNA) expression levels of 11 
      transcripts in (1) primary cultures of chicken embryonic hepatocytes (CEH; all 
      four BFRs) and (2) chicken embryos (HCDBCO and BTBPE only). Neither hepatocyte 
      viability nor embryonic pipping success were affected by the BFRs at any of the 
      administered concentrations (CEH: 0.001-30 muM, egg injection: 0.1-10 mug/g nominal 
      dose). In CEH, 10 muM HCDBCO induced cytochrome P450 2H1 (CYP2H1) and CYP3A37, 
      while CYP1A4/5 were down-regulated at all tested concentrations. In contrast, 
      only transthyretin was down-regulated by HCDBCO in embryonic liver. There was 
      concordance between the BTBPE-induced transcriptional responses in vitro and in 
      ovo for CYP1A4/5 (up-regulated) and type III iodothyronine 5'-deiodinase (DIO3; 
      down-regulated). DBDPE induced CYP1A4/5 29- and 59-fold at 0.2 muM in CEH and 
      increased DIO1. None of the gene targets were responsive to BEHTBP exposure in 
      CEH. The multi-tiered in vitro/in ovo screening approach was effective for 
      assessing toxicological and molecular biological effects of these BFRs in an 
      avian species.
CI  - Crown Copyright (c) 2011. Published by Elsevier Ireland Ltd. All rights reserved.
FAU - Egloff, Caroline
AU  - Egloff C
AD  - Centre for Advanced Research in Environmental Genomics, Department of Biology, 
      University of Ottawa, 20 Marie Curie Street, Ottawa, Ontario, Canada K1N6N5.
FAU - Crump, Doug
AU  - Crump D
FAU - Chiu, Suzanne
AU  - Chiu S
FAU - Manning, Gillian
AU  - Manning G
FAU - McLaren, Kristina K
AU  - McLaren KK
FAU - Cassone, Cristina G
AU  - Cassone CG
FAU - Letcher, Robert J
AU  - Letcher RJ
FAU - Gauthier, Lewis T
AU  - Gauthier LT
FAU - Kennedy, Sean W
AU  - Kennedy SW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110826
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
RN  - 0 (Flame Retardants)
RN  - 0 (Polybrominated Biphenyls)
RN  - 0 (RNA, Messenger)
RN  - EC 1.11.1.- (iodothyronine deiodinase type I)
RN  - EC 1.11.1.8 (Iodide Peroxidase)
RN  - EC 1.14.14.1 (Aryl Hydrocarbon Hydroxylases)
RN  - EC 1.14.14.1 (Cytochrome P450 Family 3)
RN  - EC 1.14.14.1 (cytochrome P-450 CYP3A37 (chicken))
SB  - IM
MH  - Animals
MH  - Aryl Hydrocarbon Hydroxylases/biosynthesis/genetics
MH  - Cell Survival/drug effects
MH  - Chick Embryo
MH  - Cytochrome P450 Family 3
MH  - Flame Retardants/*toxicity
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Enzymologic/*drug effects
MH  - Hepatocytes/drug effects/metabolism
MH  - Iodide Peroxidase/biosynthesis/genetics
MH  - Liver/cytology/*drug effects/embryology/enzymology
MH  - Polybrominated Biphenyls/*toxicity
MH  - RNA, Messenger/*biosynthesis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Statistics, Nonparametric
EDAT- 2011/09/07 06:00
MHDA- 2012/01/19 06:00
CRDT- 2011/09/07 06:00
PHST- 2011/07/26 00:00 [received]
PHST- 2011/08/18 00:00 [revised]
PHST- 2011/08/19 00:00 [accepted]
PHST- 2011/09/07 06:00 [entrez]
PHST- 2011/09/07 06:00 [pubmed]
PHST- 2012/01/19 06:00 [medline]
AID - S0378-4274(11)01501-3 [pii]
AID - 10.1016/j.toxlet.2011.08.015 [doi]
PST - ppublish
SO  - Toxicol Lett. 2011 Nov 10;207(1):25-33. doi: 10.1016/j.toxlet.2011.08.015. Epub 
      2011 Aug 26.