PMID- 21893610
OWN - NLM
STAT- MEDLINE
DCOM- 20120319
LR  - 20211020
IS  - 1741-7899 (Electronic)
IS  - 1470-1626 (Print)
IS  - 1470-1626 (Linking)
VI  - 142
IP  - 5
DP  - 2011 Nov
TI  - Hedgehog signalling promotes germ cell survival in the rat testis.
PG  - 711-21
LID - 10.1530/REP-11-0110 [doi]
AB  - Hedgehog (Hh) signalling has a crucial role in testis development. Sertoli 
      cell-derived desert hedgehog (DHH) guides the formation of testis cords and 
      differentiation of foetal-type Leydig cells. Dhh mutant mice are infertile due to 
      a block in germ cell differentiation, hypogonadism and hypoandrogenism. Hh 
      signalling pathway components are also expressed in postnatal testis. In the rat 
      testis the transcription factor of the Hh pathway, glioma-associated oncogene 
      homologue (GLI1), is expressed by a wide variety of germ cells. This suggests 
      that Hh signalling is involved in spermatogenesis at many different levels. Our 
      data show that canonical Hh signalling is turned off in early condensing 
      spermatids that strongly express the negative regulator of the pathway, 
      suppressor of fused (SUFU). Most of the Hh pathway specific mRNAs display the 
      highest values in stages II-VI of the rat seminiferous epithelial cycle. The key 
      endocrine regulator of germ cell differentiation, FSH, down-regulates Dhh mRNA 
      levels in vitro. Hh signalling inhibition in vitro leads to massive apoptosis of 
      germ cells. In prepubertal rat testis imatinib mesylate-induced inhibition of 
      tyrosine kinases impinges on Dhh transcript levels and Hh signalling. Our data 
      indicate that Hh signalling is part of the paracrine signalling network in the 
      rat testis. It promotes the survival of germ cells and is suppressed by FSH.
FAU - Makela, Juho-Antti
AU  - Makela JA
AD  - Department of Physiology, University of Turku, FIN-20520 Turku, Finland. 
      juho-antti.makela@utu.fi
FAU - Saario, Vuokko
AU  - Saario V
FAU - Bourguiba-Hachemi, Sonia
AU  - Bourguiba-Hachemi S
FAU - Nurmio, Mirja
AU  - Nurmio M
FAU - Jahnukainen, Kirsi
AU  - Jahnukainen K
FAU - Parvinen, Martti
AU  - Parvinen M
FAU - Toppari, Jorma
AU  - Toppari J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110905
PL  - England
TA  - Reproduction
JT  - Reproduction (Cambridge, England)
JID - 100966036
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Benzamides)
RN  - 0 (Hedgehog Proteins)
RN  - 0 (Piperazines)
RN  - 0 (Pyrimidines)
RN  - 0 (Teratogens)
RN  - 0 (Veratrum Alkaloids)
RN  - 8A1O1M485B (Imatinib Mesylate)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
RN  - ZH658AJ192 (cyclopamine)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Antineoplastic Agents/pharmacology
MH  - Benzamides
MH  - Cell Survival/drug effects/genetics
MH  - Follicle Stimulating Hormone/pharmacology
MH  - Gene Expression Regulation, Developmental/drug effects
MH  - Germ Cells/drug effects/metabolism/*physiology
MH  - Hedgehog Proteins/antagonists & inhibitors/genetics/metabolism/*physiology
MH  - Imatinib Mesylate
MH  - Male
MH  - Paracrine Communication/drug effects/genetics
MH  - Piperazines/pharmacology
MH  - Pyrimidines/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sexual Maturation/drug effects/genetics/physiology
MH  - Signal Transduction/drug effects/genetics/physiology
MH  - Teratogens/pharmacology
MH  - Testis/cytology/growth & development/*metabolism/*physiology
MH  - Veratrum Alkaloids/pharmacology
PMC - PMC3207727
EDAT- 2011/09/07 06:00
MHDA- 2012/03/20 06:00
PMCR- 2011/11/01
CRDT- 2011/09/07 06:00
PHST- 2011/09/07 06:00 [entrez]
PHST- 2011/09/07 06:00 [pubmed]
PHST- 2012/03/20 06:00 [medline]
PHST- 2011/11/01 00:00 [pmc-release]
AID - REP-11-0110 [pii]
AID - REP110110 [pii]
AID - 10.1530/REP-11-0110 [doi]
PST - ppublish
SO  - Reproduction. 2011 Nov;142(5):711-21. doi: 10.1530/REP-11-0110. Epub 2011 Sep 5.