PMID- 21894381
OWN - NLM
STAT- MEDLINE
DCOM- 20120213
LR  - 20190606
IS  - 1678-8060 (Electronic)
IS  - 0074-0276 (Linking)
VI  - 106
IP  - 5
DP  - 2011 Aug
TI  - Dengue-2 and yellow fever 17DD viruses infect human dendritic cells, resulting in 
      an induction of activation markers, cytokines and chemokines and secretion of 
      different TNF-alpha and IFN-alpha profiles.
PG  - 594-605
LID - S0074-02762011000500012 [pii]
AB  - Flaviviruses cause severe acute febrile and haemorrhagic infections, including 
      dengue and yellow fever and the pathogenesis of these infections is caused by an 
      exacerbated immune response. Dendritic cells (DCs) are targets for dengue virus 
      (DENV) and yellow fever virus (YF) replication and are the first cell population 
      to interact with these viruses during a natural infection, which leads to an 
      induction of protective immunity in humans. We studied the infectivity of DENV2 
      (strain 16681), a YF vaccine (YF17DD) and a chimeric YF17D/DENV2 vaccine in 
      monocyte-derived DCs in vitro with regard to cell maturation, activation and 
      cytokine production. Higher viral antigen positive cell frequencies were observed 
      for DENV2 when compared with both vaccine viruses. Flavivirus-infected cultures 
      exhibited dendritic cell activation and maturation molecules. CD38 expression on 
      DCs was enhanced for both DENV2 and YF17DD, whereas OX40L expression was 
      decreased as compared to mock-stimulated cells, suggesting that a T helper 1 
      profile is favoured. Tumor necrosis factor (TNF)-alpha production in cell cultures 
      was significantly higher in DENV2-infected cultures than in cultures infected 
      with YF17DD or YF17D/DENV. In contrast, the vaccines induced higher IFN-alpha levels 
      than DENV2. The differential cytokine production indicates that DENV2 results in 
      TNF induction, which discriminates it from vaccine viruses that preferentially 
      stimulate interferon expression. These differential response profiles may 
      influence the pathogenic infection outcome.
FAU - Gandini, Mariana
AU  - Gandini M
AD  - Laboratorio de Imunologia Viral, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, 
      Brasil.
FAU - Reis, Sonia Regina Nogueira Ignacio
AU  - Reis SR
FAU - Torrentes-Carvalho, Amanda
AU  - Torrentes-Carvalho A
FAU - Azeredo, Elzinandes Leal
AU  - Azeredo EL
FAU - Freire, Marcos da Silva
AU  - Freire Mda S
FAU - Galler, Ricardo
AU  - Galler R
FAU - Kubelka, Claire Fernandes
AU  - Kubelka CF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Brazil
TA  - Mem Inst Oswaldo Cruz
JT  - Memorias do Instituto Oswaldo Cruz
JID - 7502619
RN  - 0 (Biomarkers)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Dengue Vaccines)
RN  - 0 (Interferon-alpha)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Yellow Fever Vaccine)
SB  - IM
MH  - Biomarkers/analysis
MH  - Cell Differentiation
MH  - Chemokines/biosynthesis
MH  - Cytokines/*biosynthesis
MH  - Dendritic Cells/*immunology/virology
MH  - Dengue/*immunology/virology
MH  - Dengue Vaccines/immunology
MH  - Dengue Virus/*immunology/physiology
MH  - Humans
MH  - Interferon-alpha/immunology/metabolism
MH  - Tumor Necrosis Factor-alpha/immunology/metabolism
MH  - Virus Replication
MH  - Yellow Fever/*immunology/virology
MH  - Yellow Fever Vaccine/immunology
MH  - Yellow fever virus/*immunology/physiology
EDAT- 2011/09/07 06:00
MHDA- 2012/02/14 06:00
CRDT- 2011/09/07 06:00
PHST- 2011/02/07 00:00 [received]
PHST- 2011/06/08 00:00 [accepted]
PHST- 2011/09/07 06:00 [entrez]
PHST- 2011/09/07 06:00 [pubmed]
PHST- 2012/02/14 06:00 [medline]
AID - S0074-02762011000500012 [pii]
AID - 10.1590/s0074-02762011000500012 [doi]
PST - ppublish
SO  - Mem Inst Oswaldo Cruz. 2011 Aug;106(5):594-605. doi: 
      10.1590/s0074-02762011000500012.