PMID- 21895877
OWN - NLM
STAT- MEDLINE
DCOM- 20120213
LR  - 20131121
IS  - 1365-2982 (Electronic)
IS  - 1350-1925 (Linking)
VI  - 23
IP  - 11
DP  - 2011 Nov
TI  - Persistent epithelial barrier alterations in a rat model of postinfectious gut 
      dysfunction.
PG  - e523-33
LID - 10.1111/j.1365-2982.2011.01777.x [doi]
AB  - BACKGROUND: Mucosal mast cells (MMCs), epithelial barrier function (EBF) and the 
      enteric nervous system (ENS) are interactive factors in the pathophysiology of 
      functional gastrointestinal disorders. We characterized postinfectious EBF 
      alterations in the Trichinella spiralis infection model of MMC-dependent 
      intestinal dysfunction in rats. METHODS: Sprague-Dawley rats were infected with 
      T. spiralis. 30 +/- 2 days postinfection, jejunal EBF (electrophysiological 
      parameters, fluorescein isothiocyanate-dextran fluxes and responses to 
      secretagogues and MMC degranulators) was evaluated (Ussing chamber). In some 
      experiments, participation of secretomotor neurons was examined by tetrodotoxin 
      (TTX) pretreatment. Jejunal histology and MMC count and activity were also 
      assessed. KEY RESULTS: 30 +/- 2 days postinfection, when only a low grade 
      inflammation was observed, increased MMC number and activity were associated with 
      altered EBF. EBF alterations were characterized by increased mucosal permeability 
      and ion secretion. In T. spiralis-infected animals, secretory responses to 
      serotonin (5-HT) and immunoglobulin E (IgE)-dependent activation of MMCs were 
      reduced. In contrast, responses to substance P (SP) and capsaicin were similar in 
      infected and noninfected animals. Neuronal blockade with TTX altered secretory 
      responses to SP and capsaicin only in infected rats. CONCLUSIONS & INFERENCES: 
      Trichinella spiralis infection in rats, at late stages, results in persistent 
      postinfectious intestinal barrier dysfunctions and mucosal mastocytosis, with 
      other signs suggestive of a low grade inflammation. The altered permeability and 
      the TTX-independent hyporesponsiveness to 5-HT and IgE indicate epithelial 
      alterations. Changes in responses to SP and capsaicin after neuronal blockade 
      suggest an ENS remodeling during this phase. Similar long-lasting 
      neuro-epithelial alterations might contribute to the pathophysiology of 
      functional and postinfectious gastrointestinal disorders.
CI  - (c) 2011 Blackwell Publishing Ltd.
FAU - Fernandez-Blanco, J A
AU  - Fernandez-Blanco JA
AD  - Department of Cell Biology, Physiology and Immunology, Veterinary School, 
      Universidad Autonoma de Barcelona, Barcelona, Spain.
FAU - Barbosa, S
AU  - Barbosa S
FAU - Sanchez de Medina, F
AU  - Sanchez de Medina F
FAU - Martinez, V
AU  - Martinez V
FAU - Vergara, P
AU  - Vergara P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110905
PL  - England
TA  - Neurogastroenterol Motil
JT  - Neurogastroenterology and motility
JID - 9432572
RN  - 0 (Neurotransmitter Agents)
RN  - 0 (Sensory System Agents)
RN  - 0 (Sodium Channel Blockers)
RN  - 33507-63-0 (Substance P)
RN  - 4368-28-9 (Tetrodotoxin)
RN  - EC 3.4.21.- (chymase 2)
RN  - EC 3.4.21.39 (Chymases)
RN  - S07O44R1ZM (Capsaicin)
SB  - IM
MH  - Animals
MH  - Capsaicin/pharmacology
MH  - Chymases/metabolism
MH  - Enteric Nervous System/drug effects/pathology/physiopathology
MH  - Gastrointestinal Tract/microbiology/pathology/physiopathology
MH  - Inflammation/etiology/pathology/physiopathology
MH  - Intestinal Mucosa/innervation/*microbiology/*pathology/*physiopathology
MH  - Ion Transport/physiology
MH  - Male
MH  - Mast Cells/cytology/enzymology/microbiology/pathology
MH  - Mastocytosis/etiology/pathology/physiopathology
MH  - Neurotransmitter Agents/pharmacology
MH  - Permeability
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sensory System Agents/pharmacology
MH  - Sodium Channel Blockers/pharmacology
MH  - Substance P/pharmacology
MH  - Tetrodotoxin/pharmacology
MH  - Trichinella spiralis/pathogenicity
MH  - Trichinellosis/complications/*pathology/*physiopathology
EDAT- 2011/09/08 06:00
MHDA- 2012/02/14 06:00
CRDT- 2011/09/08 06:00
PHST- 2011/09/08 06:00 [entrez]
PHST- 2011/09/08 06:00 [pubmed]
PHST- 2012/02/14 06:00 [medline]
AID - 10.1111/j.1365-2982.2011.01777.x [doi]
PST - ppublish
SO  - Neurogastroenterol Motil. 2011 Nov;23(11):e523-33. doi: 
      10.1111/j.1365-2982.2011.01777.x. Epub 2011 Sep 5.