PMID- 21896156
OWN - NLM
STAT- MEDLINE
DCOM- 20120117
LR  - 20140217
IS  - 2041-1014 (Electronic)
IS  - 2041-1006 (Linking)
VI  - 26
IP  - 5
DP  - 2011 Oct
TI  - Clonal structure of Streptococcus sanguinis strains isolated from endocarditis 
      cases and the oral cavity.
PG  - 291-302
LID - 10.1111/j.2041-1014.2011.00618.x [doi]
AB  - A collection of Streptococcus sanguinis strains from patients with endocarditis 
      (n = 21) and from the oral cavity (n = 34) was subjected to a multi-locus 
      sequence typing analysis using seven housekeeping genes, carbamoyl-phosphate 
      synthetase (carB), Co/Zn/Cd efflux system component (czcD), d-alanyl-d-alanine 
      ligase (ddl), DNA polymerase III (dnaX), glucose-6-phosphate dehydrogenase (gdh), 
      DNA-directed RNA polymerase, beta subunit (rpoB) and superoxide dismutase (sodA). 
      The scheme was expanded by the inclusion of two the putative virulence genes, 
      bacitracin-resistance protein (bacA) and saliva-binding protein (ssaB), to 
      increase strain discrimination. Extensive intra-species recombination was 
      apparent in all genes but inter-species recombination was also apparent with 
      strains apparently harbouring gdh and ddl from unidentified sources and one 
      isolate harboured a sodA allele apparently derived from Streptococcus oralis. The 
      recombination/mutation ratio for the concatenated housekeeping gene sequences was 
      1.67 (95% confidence limits 1.25-2.72) and for the two virulence genes the r/m 
      ratio was 3.99 (95% confidence limits 1.61-8.72); recombination was the major 
      driver for genetic variation. All isolates were distinct and the endocarditis 
      strains did not form distinct sub-clusters when the data were analysed using 
      ClonalFrame. These data support the widely held opinion that infecting S. 
      sanguinis strains are opportunistic human pathogens.
CI  - (c) 2011 John Wiley & Sons A/S.
FAU - Do, T
AU  - Do T
AD  - Department of Microbiology, King's College London, London, UK.
FAU - Gilbert, S C
AU  - Gilbert SC
FAU - Klein, J
AU  - Klein J
FAU - Warren, S
AU  - Warren S
FAU - Wade, W G
AU  - Wade WG
FAU - Beighton, D
AU  - Beighton D
LA  - eng
GR  - Department of Health/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110705
PL  - Denmark
TA  - Mol Oral Microbiol
JT  - Molecular oral microbiology
JID - 101524770
MH  - Clone Cells
MH  - Dental Plaque/*microbiology
MH  - Endocarditis, Bacterial/blood/*microbiology
MH  - Genes, Bacterial/*genetics
MH  - Genes, Essential/genetics
MH  - Genetic Variation
MH  - Humans
MH  - Multilocus Sequence Typing
MH  - Opportunistic Infections/microbiology
MH  - Phylogeny
MH  - Recombination, Genetic
MH  - Streptococcus sanguis/*genetics
EDAT- 2011/09/08 06:00
MHDA- 2012/01/18 06:00
CRDT- 2011/09/08 06:00
PHST- 2011/09/08 06:00 [entrez]
PHST- 2011/09/08 06:00 [pubmed]
PHST- 2012/01/18 06:00 [medline]
AID - 10.1111/j.2041-1014.2011.00618.x [doi]
PST - ppublish
SO  - Mol Oral Microbiol. 2011 Oct;26(5):291-302. doi: 
      10.1111/j.2041-1014.2011.00618.x. Epub 2011 Jul 5.