PMID- 2189724
OWN - NLM
STAT- MEDLINE
DCOM- 19900706
LR  - 20220511
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 9
IP  - 6
DP  - 1990 Jun
TI  - Two distinct and frequently mutated regions of retinoblastoma protein are 
      required for binding to SV40 T antigen.
PG  - 1815-22
AB  - The retinoblastoma susceptibility gene (RB) encodes a phosphoprotein of 110 kd 
      (pp110RB) that forms specific complexes with SV40 T antigen and the transforming 
      proteins of several other DNA tumor viruses. Interaction with RB is thought to 
      contribute to transformation by these viruses as demonstrated by genetic 
      analyses. To help understand the function of these interactions, the regions of 
      RB that are involved in binding to T have been mapped. An in vitro protein 
      synthesis system capable of producing full-length RB protein has been developed 
      to facilitate the mapping study. A 5- to 10-fold increase in translational 
      efficiency in the reticulocyte lysate was obtained when the 5' non-coding region 
      of RB mRNA was replaced with that of beta-globin mRNA or a plant viral RNA, 
      alfalfa mosaic virus (AMV) RNA4. A series of mutated RB polypeptides produced 
      from this system were assayed for T binding. Two non-contiguous regions of the RB 
      protein, amino acid residues 394-571 and 649-773, were found to be necessary for 
      binding to T: mutations in either region abolished T-RB complex formation. These 
      results are consistent with the finding that, in all the cases analyzed so far, 
      mutated RB proteins in human tumor cells also failed to bind to T antigen due to 
      deletions including at least one of the two required regions. Thus the regions of 
      RB defined in vitro as necessary for interaction with T might be physiologically 
      relevant as well, and might play a fundamental role in normal RB protein 
      function.
FAU - Huang, S
AU  - Huang S
AD  - Department of Pathology, University of California at San Diego, La Jolla 92093.
FAU - Wang, N P
AU  - Wang NP
FAU - Tseng, B Y
AU  - Tseng BY
FAU - Lee, W H
AU  - Lee WH
FAU - Lee, E H
AU  - Lee EH
LA  - eng
GR  - CA 49649/CA/NCI NIH HHS/United States
GR  - EY 05758/EY/NEI NIH HHS/United States
GR  - EY 07737/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Antigens, Polyomavirus Transforming)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Retinoblastoma Protein)
RN  - 9004-22-2 (Globins)
SB  - IM
MH  - Antigens, Polyomavirus Transforming/*metabolism
MH  - Base Sequence
MH  - Chimera
MH  - Exons
MH  - Globins/biosynthesis/genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Mosaic Viruses/genetics
MH  - *Mutation
MH  - *Oncogenes
MH  - Phosphoproteins/*genetics/metabolism
MH  - Protein Biosynthesis
MH  - RNA, Messenger/biosynthesis
MH  - Restriction Mapping
MH  - Retinoblastoma/*genetics
MH  - Retinoblastoma Protein
MH  - Transcription, Genetic
MH  - Tumor Cells, Cultured
PMC - PMC551886
EDAT- 1990/06/01 00:00
MHDA- 1990/06/01 00:01
PMCR- 1991/06/01
CRDT- 1990/06/01 00:00
PHST- 1990/06/01 00:00 [pubmed]
PHST- 1990/06/01 00:01 [medline]
PHST- 1990/06/01 00:00 [entrez]
PHST- 1991/06/01 00:00 [pmc-release]
AID - 10.1002/j.1460-2075.1990.tb08306.x [doi]
PST - ppublish
SO  - EMBO J. 1990 Jun;9(6):1815-22. doi: 10.1002/j.1460-2075.1990.tb08306.x.