PMID- 21899852
OWN - NLM
STAT- MEDLINE
DCOM- 20111208
LR  - 20131121
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1218
IP  - 41
DP  - 2011 Oct 14
TI  - Coupling frontal elution paper chromatography with desorption corona beam 
      ionization mass spectrometry for rapid analysis of chlorphenamine in herbal 
      medicines and dietary supplements.
PG  - 7371-6
LID - 10.1016/j.chroma.2011.08.067 [doi]
AB  - We developed a convenient method by coupling frontal elution paper chromatography 
      with desorption corona beam ionization mass spectrometry (DCBI-MS) for rapid 
      determination of chlorphenamine added in herbal medicines or dietary supplements. 
      In this method, the ethanol extract of the herbal products was spotted directly 
      onto an isosceles triangular filter paper sheet, and then the paper sheet was 
      developed under strong elution condition with the sample zone migrating at the 
      solvent front. The analyte was finally condensed at the V-shaped tip which could 
      then be placed under the visible plasma beam of DCBI for ionization. The overall 
      procedure took less than 5 min. The frontal elution paper chromatography on a 
      triangular plate used in this work improved the signal intensity of 
      chlorphenamine by 30-fold due to the analyte condensing at the tip and the 
      reduction of the background suppression. Furthermore, the paper sheet also 
      functioned as a filter in the analysis of solid or powder samples, which can 
      increase the analytical throughput by omitting the step of centrifugation. The 
      proposed method in current study was successfully applied in the determination of 
      chlorphenamine in herbal medicines. Chlorphenamine was detected in four of the 
      twelve types of herbal medicines examined in this study. The limit of detection 
      was 200 ng/mL (2.0 ng absolute) in full-scan positive-ion mode and the linear 
      range was from 5.0 mug/mL to 50 mug/mL with satisfactory linear coefficient (R(2) 
      (the square of the correlation coefficient)=0.895). Good reproducibility was 
      achieved with relative standard deviations (RSDs) less than 15.0% and the 
      recoveries of chlorphenamine ranged from 84.3 to 90.6%.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Huang, Yun-Qing
AU  - Huang YQ
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, China.
FAU - You, Jing-Qing
AU  - You JQ
FAU - Zhang, Junsheng
AU  - Zhang J
FAU - Sun, Wenjian
AU  - Sun W
FAU - Ding, Li
AU  - Ding L
FAU - Feng, Yu-Qi
AU  - Feng YQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110825
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Tablets)
RN  - 3K9958V90M (Ethanol)
RN  - 3U6IO1965U (Chlorpheniramine)
SB  - IM
MH  - Chlorpheniramine/*analysis
MH  - Chromatography, Liquid/*methods
MH  - Dietary Supplements/*analysis
MH  - Drugs, Chinese Herbal/*chemistry
MH  - Ethanol
MH  - Linear Models
MH  - Mass Spectrometry/*methods
MH  - Paper
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Tablets/chemistry
EDAT- 2011/09/09 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/09/09 06:00
PHST- 2011/06/30 00:00 [received]
PHST- 2011/08/22 00:00 [revised]
PHST- 2011/08/22 00:00 [accepted]
PHST- 2011/09/09 06:00 [entrez]
PHST- 2011/09/09 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - S0021-9673(11)01277-5 [pii]
AID - 10.1016/j.chroma.2011.08.067 [doi]
PST - ppublish
SO  - J Chromatogr A. 2011 Oct 14;1218(41):7371-6. doi: 10.1016/j.chroma.2011.08.067. 
      Epub 2011 Aug 25.