PMID- 21900530
OWN - NLM
STAT- MEDLINE
DCOM- 20120221
LR  - 20220316
IS  - 1556-679X (Electronic)
IS  - 1556-6811 (Print)
IS  - 1556-679X (Linking)
VI  - 18
IP  - 11
DP  - 2011 Nov
TI  - Outer membrane vesicles induce immune responses to virulence proteins and protect 
      against colonization by enterotoxigenic Escherichia coli.
PG  - 1803-8
LID - 10.1128/CVI.05217-11 [doi]
AB  - Enterotoxigenic Escherichia coli (ETEC) strains are a heterogeneous group of 
      pathogens that produce heat-labile (LT) and/or heat-stable (ST) enterotoxins. 
      Collectively, these pathogens are responsible for hundreds of thousands of deaths 
      annually in developing countries, particularly in children under the age of 5 
      years. The heterogeneity of previously investigated molecular targets and the 
      lack of complete sustained protection afforded by antitoxin immunity have impeded 
      progress to date toward a broadly protective vaccine. Many pathogens, including 
      ETEC, have the capacity to form outer membrane vesicles (OMV), which often 
      contain one or more virulence proteins. Prompted by recent studies that 
      identified several immunogenic virulence proteins in outer membrane vesicles of 
      ETEC, we sought to examine the immunogenicity and protective efficacy of these 
      structures in a murine model of infection. Here we demonstrate that immunization 
      with OMV impairs ETEC colonization of the small intestine and stimulates 
      antibodies that recognize the heat-labile toxin and two additional putative 
      virulence proteins, the EtpA adhesin and CexE. Similar to earlier studies with 
      EtpA, vaccination with LT alone also inhibited intestinal colonization. Together, 
      these findings suggest that OMV could be exploited to deliver protective antigens 
      relevant to development of ETEC vaccines.
FAU - Roy, Koushik
AU  - Roy K
AD  - Department of Medicine, University of Tennessee Health Sciences Center, Memphis, 
      Tennessee, USA.
FAU - Hamilton, David J
AU  - Hamilton DJ
FAU - Munson, George P
AU  - Munson GP
FAU - Fleckenstein, James M
AU  - Fleckenstein JM
LA  - eng
GR  - I01 BX001469/BX/BLRD VA/United States
GR  - R01 AI089894/AI/NIAID NIH HHS/United States
GR  - R01 AI089894-01/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20110907
PL  - United States
TA  - Clin Vaccine Immunol
JT  - Clinical and vaccine immunology : CVI
JID - 101252125
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Bacterial Toxins)
RN  - 0 (Enterotoxins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Escherichia coli Vaccines)
RN  - 0 (Virulence Factors)
RN  - 0 (heat stable toxin (E coli))
RN  - D9K3SN2LNY (heat-labile enterotoxin, E coli)
SB  - IM
MH  - Animals
MH  - Antibodies, Bacterial/blood
MH  - Bacterial Toxins/*immunology
MH  - Enterotoxigenic Escherichia coli/*immunology/pathogenicity
MH  - Enterotoxins/*immunology
MH  - Escherichia coli Infections/immunology/*prevention & control
MH  - Escherichia coli Proteins/*immunology
MH  - Escherichia coli Vaccines/administration & dosage/*immunology
MH  - Exosomes/*immunology
MH  - Intestine, Small/microbiology
MH  - Mice
MH  - Virulence Factors/*immunology
PMC - PMC3209013
EDAT- 2011/09/09 06:00
MHDA- 2012/02/22 06:00
PMCR- 2012/05/01
CRDT- 2011/09/09 06:00
PHST- 2011/09/09 06:00 [entrez]
PHST- 2011/09/09 06:00 [pubmed]
PHST- 2012/02/22 06:00 [medline]
PHST- 2012/05/01 00:00 [pmc-release]
AID - CVI.05217-11 [pii]
AID - 5217-11 [pii]
AID - 10.1128/CVI.05217-11 [doi]
PST - ppublish
SO  - Clin Vaccine Immunol. 2011 Nov;18(11):1803-8. doi: 10.1128/CVI.05217-11. Epub 
      2011 Sep 7.