PMID- 21902606
OWN - NLM
STAT- MEDLINE
DCOM- 20120426
LR  - 20220321
IS  - 1937-335X (Electronic)
IS  - 1937-3341 (Linking)
VI  - 18
IP  - 1-2
DP  - 2012 Jan
TI  - Differentiation of human Wharton's jelly cells toward nucleus pulposus-like cells 
      after coculture with nucleus pulposus cells in vitro.
PG  - 167-75
LID - 10.1089/ten.TEA.2011.0186 [doi]
AB  - The aim of this study was to evaluate whether human Wharton's jelly cells (WJCs) 
      could be differentiated into nucleus pulposus (NP)-like cells by coculturing with 
      NP cells (NPCs) in vitro. WJCs were isolated from the human umbilical cord, and 
      NPCs were isolated from healthy human intervertebral disc. After coculturing WJCs 
      with NPCs in a monolayer environment with or without cell-cell contact for 7 
      days, the real-time polymerase chain reaction showed the relative gene 
      expressions of NP-marker genes (aggrecan, type II collagens, and SRY-type HMG 
      box-9) were significantly increased (p<0.05) in all groups, and the increase in 
      the group of 25:75/WJCs:NPCs was the largest (p<0.05). The increases of relative 
      gene expression in WJCs cocultured with cell-cell contact were larger than those 
      cocultured without contact in all ratios (p<0.05). WJCs were positive for 
      telomerase expression. Flow cytometry analyses showed that WJCs expressed CD73, 
      CD105, CD90, CD29, CD166, and human leukocyte antigen (HLA)-ABC while being 
      negative for the expression of CD34, CD45, and HLA-DR. The results of this study 
      indicated that the WJCs had the feature of the mesenchymal stem cell and might be 
      induced to differentiate to NP-like cells by coculturing with NPCs.
FAU - Ruan, Dike
AU  - Ruan D
AD  - Department of Orthopaedic Surgery, Navy General Hospital, Beijing, China. 
      ruandike@yahoo.com.cn
FAU - Zhang, Yan
AU  - Zhang Y
FAU - Wang, Deli
AU  - Wang D
FAU - Zhang, Chao
AU  - Zhang C
FAU - Wu, Jianhong
AU  - Wu J
FAU - Wang, Chaofeng
AU  - Wang C
FAU - Shi, Zhiyuan
AU  - Shi Z
FAU - Xin, Hongkui
AU  - Xin H
FAU - Xu, Chen
AU  - Xu C
FAU - Li, Haifeng
AU  - Li H
FAU - He, Qing
AU  - He Q
LA  - eng
PT  - Journal Article
DEP - 20111003
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
RN  - 0 (5-(6)-carboxyfluorescein diacetate succinimidyl ester)
RN  - 0 (Biomarkers)
RN  - 0 (Fluoresceins)
RN  - 0 (Succinimides)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Adult
MH  - Biomarkers/metabolism
MH  - Cell Communication
MH  - Cell Count
MH  - *Cell Differentiation
MH  - Coculture Techniques/*methods
MH  - Flow Cytometry
MH  - Fluoresceins/metabolism
MH  - Gene Expression Regulation
MH  - Humans
MH  - Intervertebral Disc/*cytology
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology/enzymology
MH  - Staining and Labeling
MH  - Succinimides/metabolism
MH  - Telomerase/metabolism
EDAT- 2011/09/10 06:00
MHDA- 2012/04/27 06:00
CRDT- 2011/09/10 06:00
PHST- 2011/09/10 06:00 [entrez]
PHST- 2011/09/10 06:00 [pubmed]
PHST- 2012/04/27 06:00 [medline]
AID - 10.1089/ten.TEA.2011.0186 [doi]
PST - ppublish
SO  - Tissue Eng Part A. 2012 Jan;18(1-2):167-75. doi: 10.1089/ten.TEA.2011.0186. Epub 
      2011 Oct 3.