PMID- 21902673
OWN - NLM
STAT- MEDLINE
DCOM- 20120425
LR  - 20240109
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Linking)
VI  - 36
IP  - 2
DP  - 2012 Feb
TI  - (-)-Epigallocatechin gallate suppresses adipocyte differentiation through the 
      MEK/ERK and PI3K/Akt pathways.
PG  - 147-53
LID - 10.1042/CBI20110047 [doi]
AB  - EGCG [(-)-epigallocatechin gallate], tea catechin, is one of the compounds that 
      has been reported to act against obesity and diabetes. To determine the effect of 
      EGCG on adipocyte differentiation, we treated 3T3-L1 preadipocytes with different 
      catechins. Oil Red O staining showed significantly reduced intracellular lipid 
      accumulation, especially with EGCG. Cell cycle analysis showed that EGCG 
      inhibited cell proliferation by disturbing the cell cycle during the clonal 
      expansion of 3T3-L1. RT-PCR (real-time PCR) demonstrated that EGCG noticeably 
      reduced mRNA expression of PPARgamma (peroxisome proliferator-activated receptor gamma), 
      C/EBPalpha (CCAAT/enhancer-binding protein alpha) and FoxO1 (forkhead box class O1). EGCG 
      also caused a significant decrease in the transcription of FoxO1 - the forkhead 
      transcription factor class O1 involved in adipocyte differentiation - via the 
      PI3K (phosphoinositide 3-kinase)/Akt and MEK [MAPK (mitogen-activated protein 
      kinase)/ERK (extracellular-signal-regulated kinase) kinase] pathways. These 
      results suggest that EGCG suppresses the clonal expansion of adipocytes by 
      inactivating FoxO1 via insulin signalling and stress-dependent MAPK pathways.
FAU - Kim, Hyojung
AU  - Kim H
AD  - Graduate School of Life and Environmental Sciences, University of Tsukuba, 
      Ibaraki, Japan.
FAU - Sakamoto, Kazuichi
AU  - Sakamoto K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (CCAAT-Enhancer-Binding Protein-alpha)
RN  - 0 (Forkhead Box Protein O1)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Foxo1 protein, mouse)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (PPAR gamma)
RN  - 8R1V1STN48 (Catechin)
RN  - BQM438CTEL (epigallocatechin gallate)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/*cytology
MH  - Animals
MH  - CCAAT-Enhancer-Binding Protein-alpha/genetics/metabolism
MH  - Catechin/*analogs & derivatives/pharmacology
MH  - Cell Differentiation/*drug effects
MH  - Cell Proliferation
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Forkhead Box Protein O1
MH  - Forkhead Transcription Factors/genetics/metabolism
MH  - Hypoglycemic Agents/pharmacology
MH  - Mice
MH  - Mitogen-Activated Protein Kinase Kinases/metabolism
MH  - PPAR gamma/genetics/metabolism
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - S Phase
MH  - Signal Transduction/*drug effects
MH  - Transcription, Genetic
EDAT- 2011/09/10 06:00
MHDA- 2012/04/26 06:00
CRDT- 2011/09/10 06:00
PHST- 2011/09/10 06:00 [entrez]
PHST- 2011/09/10 06:00 [pubmed]
PHST- 2012/04/26 06:00 [medline]
AID - CBI20110047 [pii]
AID - 10.1042/CBI20110047 [doi]
PST - ppublish
SO  - Cell Biol Int. 2012 Feb;36(2):147-53. doi: 10.1042/CBI20110047.