PMID- 21903125
OWN - NLM
STAT- MEDLINE
DCOM- 20120113
LR  - 20141120
IS  - 1096-0333 (Electronic)
IS  - 0041-008X (Linking)
VI  - 257
IP  - 1
DP  - 2011 Nov 15
TI  - The effects of levofloxacin on rabbit anterior cruciate ligament cells in vitro.
PG  - 67-73
LID - 10.1016/j.taap.2011.08.019 [doi]
AB  - Articular cartilage, epiphyseal growth plate and tendons have been recognized as 
      targets of fluoroquinolone-induced connective tissue toxicity. The effects of 
      fluoroquinolones on ligament tissues are still unknown. The aim of this study was 
      to investigate the effects of levofloxacin, a typical fluoroquinolone antibiotic 
      drug, on rabbit anterior cruciate ligament (ACL) cells in vitro. Rabbit ACL cells 
      were treated with levofloxacin at different concentrations (0, 14, 28, 56, 112 
      and 224 muM) and were assessed to determine the possible cytotoxic effects of 
      levofloxacin on ACL cells. Levofloxacin, with concentrations ranging from 28 to 
      224 muM, induced dose-dependent ACL cell apoptosis. Characteristic markers of 
      programmed cell death and degenerative changes were identified by electron 
      microscopy in the ACL cells treated with 28 muM of levofloxacin. Moreover, 
      levofloxacin significantly increased the mRNA expression of matrix 
      metalloproteinase 3 (MMP-3) and MMP-13 and decreased the expression of tissue 
      inhibitors of metalloproteinase 1 (TIMP-1) in a concentration-dependent manner; 
      TIMP-3 and collagen type I alpha 1 (Col1A1) mRNA expression was not affected. 
      Immunocytochemical analysis indicated that levofloxacin markedly increased the 
      expression of active caspase-3 within a concentration range of 28 to 224 muM, 
      whereas a clear-cut decrease in Col1A1 expression was found with levofloxacin 
      treatment concentrations of 112 and 224 muM, compared to controls. Our data 
      suggest that levofloxacin has cytotoxic effects on ACL cells characterized by 
      enhanced apoptosis and decreased extracellular matrix, which suggest a potential 
      adverse effect of fluoroquinolones.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Deng, Yu
AU  - Deng Y
AD  - Department of Orthopedic Surgery, Zhongnan Hospital of Wuhan University, Wuhan, 
      China.
FAU - Chen, Biao
AU  - Chen B
FAU - Qi, Yongjian
AU  - Qi Y
FAU - Magdalou, Jacques
AU  - Magdalou J
FAU - Wang, Hui
AU  - Wang H
FAU - Chen, Liaobin
AU  - Chen L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110827
PL  - United States
TA  - Toxicol Appl Pharmacol
JT  - Toxicology and applied pharmacology
JID - 0416575
RN  - 0 (Anti-Bacterial Agents)
RN  - 6GNT3Y5LMF (Levofloxacin)
RN  - A4P49JAZ9H (Ofloxacin)
SB  - IM
MH  - Animals
MH  - Anterior Cruciate Ligament/cytology/*drug effects/ultrastructure
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - In Vitro Techniques
MH  - *Levofloxacin
MH  - Microscopy, Electron, Transmission
MH  - Ofloxacin/*pharmacology
MH  - Rabbits
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2011/09/10 06:00
MHDA- 2012/01/14 06:00
CRDT- 2011/09/10 06:00
PHST- 2011/07/17 00:00 [received]
PHST- 2011/08/15 00:00 [revised]
PHST- 2011/08/19 00:00 [accepted]
PHST- 2011/09/10 06:00 [entrez]
PHST- 2011/09/10 06:00 [pubmed]
PHST- 2012/01/14 06:00 [medline]
AID - S0041-008X(11)00333-4 [pii]
AID - 10.1016/j.taap.2011.08.019 [doi]
PST - ppublish
SO  - Toxicol Appl Pharmacol. 2011 Nov 15;257(1):67-73. doi: 
      10.1016/j.taap.2011.08.019. Epub 2011 Aug 27.